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Appl Environ Microbiol. 1982 Apr;43(4):806-9. doi: 10.1128/aem.43.4.806-809.1982.

Respiration of tritiated substrates in heterotrophic activity assays.

Applied and environmental microbiology

J Kuparinen, T Tamminen

Affiliations

  1. University of Helsinki, Tvärminne Zoological Station, SF-10850 Tvärminne, Finland.

PMID: 16345990 PMCID: PMC241922 DOI: 10.1128/aem.43.4.806-809.1982

Abstract

The respiration percentage of H-labeled glucose by heterotrophic microorganisms in water samples was determined by a method based on equimolar addition of [H]- and [C]glucose to samples. The respiration percentage of [H]glucose exceeded that of [C]glucose by 10% over the assayed range of respiration rates. Respiration percentages diminished with decreasing temperature, the relation between respiration of [H]- and [C]glucose remaining the same. The significance of tritiated water respiration with regard to experimental design is emphasized.

References

  1. Appl Environ Microbiol. 1978 Feb;35(2):456-8 - PubMed
  2. Can J Microbiol. 1967 Sep;13(9):1165-73 - PubMed

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