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Ferguson TJ, Mah RA. Isolation and characterization of an h(2)-oxidizing thermophilic methanogen. Appl Environ Microbiol. 1983;45(1):265-74doi: 10.1128/aem.45.1.265-274.1983.
Ferguson, T. J., & Mah, R. A. (1983). Isolation and characterization of an h(2)-oxidizing thermophilic methanogen. Applied and environmental microbiology, 45(1), 265-74. https://doi.org/10.1128/aem.45.1.265-274.1983
Ferguson, T J, and Mah, R A. "Isolation and characterization of an h(2)-oxidizing thermophilic methanogen." Applied and environmental microbiology vol. 45,1 (1983): 265-74. doi: https://doi.org/10.1128/aem.45.1.265-274.1983
Ferguson TJ, Mah RA. Isolation and characterization of an h(2)-oxidizing thermophilic methanogen. Appl Environ Microbiol. 1983 Jan;45(1):265-74. doi: 10.1128/aem.45.1.265-274.1983. PMID: 16346171; PMCID: PMC242264.
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Appl Environ Microbiol. 1983 Jan;45(1):265-74. doi: 10.1128/aem.45.1.265-274.1983.

Isolation and characterization of an h(2)-oxidizing thermophilic methanogen.

Applied and environmental microbiology

T J Ferguson, R A Mah

Affiliations

  1. Division of Environmental and Nutritional Sciences, School of Public Health, University of California, Los Angeles, California 90024.

PMID: 16346171 PMCID: PMC242264 DOI: 10.1128/aem.45.1.265-274.1983

Abstract

A thermophilic methanogen was isolated from enrichment cultures originally inoculated with sludge from an anaerobic kelp digester (55 degrees C). This isolate exhibited a temperature optimum of 55 to 60 degrees C and a maximum near 70 degrees C. Growth occurred throughout the pH range of 5.5 to 9.0, with optimal growth near pH 7.2. Although 4% salt was present in the isolation medium, salt was not required for optimal growth. The thermophile utilized formate or H(2)-CO(2) but not acetate, methanol, or methylamines for growth and methanogenesis. Growth in complex medium was very rapid, and a minimum doubling time of 1.8 h was recorded in media supplemented with rumen fluid. Growth in defined media required the addition of acetate and an unknown factor(s) from digester supernatant, rumen fluid, or Trypticase. Cells in liquid culture were oval to coccoid, 0.7 to 1.8 mum in diameter, often occurring in pairs. The cells were easily lysed upon exposure to oxygen or 0.08 mg of sodium dodecyl sulfate per ml. The isolate was sensitive to tetracycline and chloramphenicol but not penicillin G or cycloserine. The DNA base composition was 59.69 mol% guanine plus cytosine.

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