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Manian SS, Gumbleton R, Buckley AM, et al. Nitrogen Fixation and Carbon Dioxide Assimilation in Rhizobium japonicum. Appl Environ Microbiol. 1984;48(2):276-9doi: 10.1128/aem.48.2.276-279.1984.
Manian, S. S., Gumbleton, R., Buckley, A. M., & O'gara, F. (1984). Nitrogen Fixation and Carbon Dioxide Assimilation in Rhizobium japonicum. Applied and environmental microbiology, 48(2), 276-9. https://doi.org/10.1128/aem.48.2.276-279.1984
Manian, S S, et al. "Nitrogen Fixation and Carbon Dioxide Assimilation in Rhizobium japonicum." Applied and environmental microbiology vol. 48,2 (1984): 276-9. doi: https://doi.org/10.1128/aem.48.2.276-279.1984
Manian SS, Gumbleton R, Buckley AM, O'gara F. Nitrogen Fixation and Carbon Dioxide Assimilation in Rhizobium japonicum. Appl Environ Microbiol. 1984 Aug;48(2):276-9. doi: 10.1128/aem.48.2.276-279.1984. PMID: 16346608; PMCID: PMC241502.
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Appl Environ Microbiol. 1984 Aug;48(2):276-9. doi: 10.1128/aem.48.2.276-279.1984.

Nitrogen Fixation and Carbon Dioxide Assimilation in Rhizobium japonicum.

Applied and environmental microbiology

S S Manian, R Gumbleton, A M Buckley, F O'gara

Affiliations

  1. Department of Microbiology, University College, Cork, Ireland.

PMID: 16346608 PMCID: PMC241502 DOI: 10.1128/aem.48.2.276-279.1984

Abstract

In free-living Rhizobium japonicum cultures, the stimulatory effect of CO(2) on nitrogenase (acetylene reduction) activity was mediated through ribulose bisphosphate carboxylase activity. Two mutant strains (CJ5 and CJ6) of R. japonicum defective in CO(2) fixation were isolated by mitomycin C treatment. No ribulose bisphosphate carboxylase activity could be detected in strain CJ6, but a low level of enzyme activity was present in strain CJ5. Mutant strain CJ5 also exhibited pleiotropic effects on carbon metabolism. The mutant strains possessed reduced levels of hydrogen uptake, formate dehydrogenase, and phosphoribulokinase activities, which indicated a regulatory relationship between these enzymes. The CO(2)-dependent stimulation of nitrogenase activity was not observed in the mutant strains. Both mutant strains nodulated soybean plants and fixed nitrogen at rates comparable to that of the wild-type strain.

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