Display options
Cite
Paul JH, Jeffrey WH. Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica. Appl Environ Microbiol. 1985;50(2):431-7doi: 10.1128/aem.50.2.431-437.1985.
Paul, J. H., & Jeffrey, W. H. (1985). Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica. Applied and environmental microbiology, 50(2), 431-7. https://doi.org/10.1128/aem.50.2.431-437.1985
Paul, J H, and Jeffrey, W H. "Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica." Applied and environmental microbiology vol. 50,2 (1985): 431-7. doi: https://doi.org/10.1128/aem.50.2.431-437.1985
Paul JH, Jeffrey WH. Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica. Appl Environ Microbiol. 1985 Aug;50(2):431-7. doi: 10.1128/aem.50.2.431-437.1985. PMID: 16346861; PMCID: PMC238638.
Copy Download .nbib Format: NLM
Share it on

Appl Environ Microbiol. 1985 Aug;50(2):431-7. doi: 10.1128/aem.50.2.431-437.1985.

Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica.

Applied and environmental microbiology

J H Paul, W H Jeffrey

Affiliations

  1. Department of Marine Science, University of South Florida, St. Petersburg, Florida 33701.

PMID: 16346861 PMCID: PMC238638 DOI: 10.1128/aem.50.2.431-437.1985

Abstract

The proteolytic enzymes pronase, trypsin, and chymotrypsin and the surfactant Triton X-100 inhibited attachment of Vibrio proteolytica to the hydrophobic substratum polystyrene by >97%. These treatments had no effect on attachment to hydrophilic substrata such as glass or tissue culture dishes. Both pronase and Triton X-100 effected the removal of previously attached cells from polystyrene but not from hydrophilic surfaces. Removal of cells from polystyrene by pronase left material (which we have termed footprints) that stained with the protein-specific stain Hoechst 2495 but not with the DNA-specific stain Hoechst 33342. Pronase treatment also caused a significant decrease in cell surface hydrophobicity as determined by phase partitioning in hexane or petroleum ether. Collectively, these results imply the existence of separate mechanisms for the adhesion of V. proteolytica to hydrophilic and hydrophobic substrata and suggest a role for protein in the latter mechanism.

References

  1. Appl Microbiol. 1972 Sep;24(3):363-8 - PubMed
  2. Arch Mikrobiol. 1973 Apr 8;91(1):29-40 - PubMed
  3. J Bacteriol. 1984 Jan;157(1):64-72 - PubMed
  4. Appl Environ Microbiol. 1984 Nov;48(5):924-9 - PubMed
  5. Infect Immun. 1983 Nov;42(2):831-4 - PubMed
  6. Arch Microbiol. 1981 Jan;128(3):267-70 - PubMed
  7. Appl Environ Microbiol. 1981 Nov;42(5):751-7 - PubMed
  8. Appl Environ Microbiol. 1982 Jun;43(6):1393-9 - PubMed
  9. Appl Environ Microbiol. 1982 Jul;44(1):184-92 - PubMed
  10. Appl Environ Microbiol. 1984 Jan;47(1):135-43 - PubMed
  11. Can J Microbiol. 1977 Dec;23(12):1733-6 - PubMed
  12. J Bacteriol. 1969 Jul;99(1):316-25 - PubMed
  13. J Bacteriol. 1984 Feb;157(2):678-80 - PubMed
  14. Vet Microbiol. 1983 Oct;8(5):443-58 - PubMed
  15. Appl Environ Microbiol. 1979 Jan;37(1):67-72 - PubMed
  16. Appl Environ Microbiol. 1982 Apr;43(4):939-44 - PubMed
  17. Appl Environ Microbiol. 1983 Aug;46(2):338-43 - PubMed
  18. Appl Environ Microbiol. 1984 Sep;48(3):497-503 - PubMed

Publication Types