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Christensen BE, Kjosbakken J, Smidsrød O. Partial Chemical and Physical Characterization of Two Extracellular Polysaccharides Produced by Marine, Periphytic Pseudomonas sp. Strain NCMB 2021. Appl Environ Microbiol. 1985;50(4):837-45doi: 10.1128/aem.50.4.837-845.1985.
Christensen, B. E., Kjosbakken, J., & Smidsrød, O. (1985). Partial Chemical and Physical Characterization of Two Extracellular Polysaccharides Produced by Marine, Periphytic Pseudomonas sp. Strain NCMB 2021. Applied and environmental microbiology, 50(4), 837-45. https://doi.org/10.1128/aem.50.4.837-845.1985
Christensen, B E, et al. "Partial Chemical and Physical Characterization of Two Extracellular Polysaccharides Produced by Marine, Periphytic Pseudomonas sp. Strain NCMB 2021." Applied and environmental microbiology vol. 50,4 (1985): 837-45. doi: https://doi.org/10.1128/aem.50.4.837-845.1985
Christensen BE, Kjosbakken J, Smidsrød O. Partial Chemical and Physical Characterization of Two Extracellular Polysaccharides Produced by Marine, Periphytic Pseudomonas sp. Strain NCMB 2021. Appl Environ Microbiol. 1985 Oct;50(4):837-45. doi: 10.1128/aem.50.4.837-845.1985. PMID: 16346916; PMCID: PMC291757.
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Appl Environ Microbiol. 1985 Oct;50(4):837-45. doi: 10.1128/aem.50.4.837-845.1985.

Partial Chemical and Physical Characterization of Two Extracellular Polysaccharides Produced by Marine, Periphytic Pseudomonas sp. Strain NCMB 2021.

Applied and environmental microbiology

B E Christensen, J Kjosbakken, O Smidsrød

Affiliations

  1. Department of Marine Biochemistry, The Norwegian Institute of Technology, N-7034 Trondheim-NTH, Norway.

PMID: 16346916 PMCID: PMC291757 DOI: 10.1128/aem.50.4.837-845.1985

Abstract

The marine bacterium Pseudomonas sp. strain NCMB 2021, which can attach to solid, and especially hydrophobic, surfaces, elaborates two different extracellular polysaccharides in batch cultures. One (polysaccharide A) was produced only during exponential growth and contained glucose, galactose, glucuronic acid, and galacturonic acid in a molar ratio of 1.00:0.81:0.42:0.32. It produced viscous solutions, formed gels at high concentrations, and precipitated with several multivalent cations. The other (polysaccharide B) was released at the end of the exponential phase and in the stationary phase. It contained equimolar amounts of N-acetylglucosamine, 2-keto-3-deoxyoctulosonic acid, an unidentified 6-deoxyhexose, and also O-acetyl groups. Despite its high molecular weight (10 to 10 as judged by gel filtration), the polysaccharide produced aqueous solutions with very low viscosities and was also soluble in 90% aqueous phenol, 80% methanol, and 80% ethanol.

References

  1. Arch Microbiol. 1983 Dec;136(4):291-6 - PubMed
  2. Crit Rev Microbiol. 1983;10(2):173-201 - PubMed
  3. Biochim Biophys Acta. 1981 Nov 5;677(3-4):471-6 - PubMed
  4. Can J Biochem. 1964 Oct;42:1403-13 - PubMed
  5. Biochem J. 1959 Sep;73:127-32 - PubMed
  6. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  7. Appl Environ Microbiol. 1979 Jan;37(1):67-72 - PubMed
  8. Appl Environ Microbiol. 1982 Jul;44(1):165-70 - PubMed
  9. Appl Environ Microbiol. 1983 Jan;45(1):64-70 - PubMed
  10. J Gen Microbiol. 1977 Sep;102(1):13-21 - PubMed
  11. Appl Environ Microbiol. 1979 Mar;37(3):414-20 - PubMed
  12. Sci Am. 1978 Jan;238(1):86-95 - PubMed
  13. J Med Genet. 1976 Apr;13(2):149-51 - PubMed
  14. J Biol Chem. 1971 Mar 25;246(6):1793-8 - PubMed
  15. Biochem Biophys Res Commun. 1974 Nov 27;61(2):489-93 - PubMed
  16. J Chromatogr. 1966 Jan;21(1):163-4 - PubMed
  17. Biopolymers. 1971;10(7):1213-27 - PubMed
  18. Carbohydr Res. 1983 Aug 16;120:131-41 - PubMed
  19. Scand J Infect Dis Suppl. 1982;33:32-6 - PubMed

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