Display options
Cite
Hankinson TR, Schmidt EL. An acidophilic and a neutrophilic nitrobacter strain isolated from the numerically predominant nitrite-oxidizing population of an Acid forest soil. Appl Environ Microbiol. 1988;54(6):1536-40doi: 10.1128/aem.54.6.1536-1540.1988.
Hankinson, T. R., & Schmidt, E. L. (1988). An acidophilic and a neutrophilic nitrobacter strain isolated from the numerically predominant nitrite-oxidizing population of an Acid forest soil. Applied and environmental microbiology, 54(6), 1536-40. https://doi.org/10.1128/aem.54.6.1536-1540.1988
Hankinson, T R, and Schmidt, E L. "An acidophilic and a neutrophilic nitrobacter strain isolated from the numerically predominant nitrite-oxidizing population of an Acid forest soil." Applied and environmental microbiology vol. 54,6 (1988): 1536-40. doi: https://doi.org/10.1128/aem.54.6.1536-1540.1988
Hankinson TR, Schmidt EL. An acidophilic and a neutrophilic nitrobacter strain isolated from the numerically predominant nitrite-oxidizing population of an Acid forest soil. Appl Environ Microbiol. 1988 Jun;54(6):1536-40. doi: 10.1128/aem.54.6.1536-1540.1988. PMID: 16347664; PMCID: PMC202692.
Copy Download .nbib Format: NLM
Share it on

Appl Environ Microbiol. 1988 Jun;54(6):1536-40. doi: 10.1128/aem.54.6.1536-1540.1988.

An acidophilic and a neutrophilic nitrobacter strain isolated from the numerically predominant nitrite-oxidizing population of an Acid forest soil.

Applied and environmental microbiology

T R Hankinson, E L Schmidt

Affiliations

  1. Department of Microbiology and Department of Soil Science, University of Minnesota, St. Paul, Minnesota 55108.

PMID: 16347664 PMCID: PMC202692 DOI: 10.1128/aem.54.6.1536-1540.1988

Abstract

Two physiologically and serologically distinct strains of chemoautotrophic nitrite-oxidizing bacteria were isolated as numerically predominant members of the nitrite-oxidizer population of an undisturbed forest soil with a pH range of 4.3 to 5.2. One isolate responded as a neutrophile, characteristic of the family Nitrobacteraceae, and cross-reacted strongly with fluorescent antibody to Nitrobacter strain Engel. The second isolate responded as an acidophile in pure culture, demonstrated maximal nitrite oxidation activity at pH 5.5, and had a pH tolerance range of pH 4.1 to 7.2. Nitrite oxidase in whole cells of the acidophile sustained activity to at least pH 3.5. Cell morphology of both strains typified the genus Nitrobacter in all respects when cultured at pH 7. However, under more acidic conditions the acidophile tended to elongate and at times appeared to branch. These data provide the first evidence for the existence of an acidophilic chemoautotrophic nitrifying bacterium. Isolation of the neutrophilic Nitrobacter strain reported here complements the earlier isolation of a neutrophilic Nitrosospira strain to provide further evidence of a prominent acid-intolerant population of chemoautotrophic nitrifiers in this acid forest soil.

References

  1. J Appl Bacteriol. 1977 Apr;42(2):253-7 - PubMed
  2. Appl Environ Microbiol. 1978 Oct;36(4):584-8 - PubMed
  3. Appl Environ Microbiol. 1986 Nov;52(5):1107-11 - PubMed
  4. Appl Environ Microbiol. 1984 Apr;47(4):607-12 - PubMed
  5. Appl Environ Microbiol. 1984 Oct;48(4):802-6 - PubMed
  6. Appl Environ Microbiol. 1981 Apr;41(4):1069-71 - PubMed

Publication Types