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Bhat S, Wallace RJ, Orskov ER. Adhesion of cellulolytic ruminal bacteria to barley straw. Appl Environ Microbiol. 1990;56(9):2698-703doi: 10.1128/aem.56.9.2698-2703.1990.
Bhat, S., Wallace, R. J., & Orskov, E. R. (1990). Adhesion of cellulolytic ruminal bacteria to barley straw. Applied and environmental microbiology, 56(9), 2698-703. https://doi.org/10.1128/aem.56.9.2698-2703.1990
Bhat, S, et al. "Adhesion of cellulolytic ruminal bacteria to barley straw." Applied and environmental microbiology vol. 56,9 (1990): 2698-703. doi: https://doi.org/10.1128/aem.56.9.2698-2703.1990
Bhat S, Wallace RJ, Orskov ER. Adhesion of cellulolytic ruminal bacteria to barley straw. Appl Environ Microbiol. 1990 Sep;56(9):2698-703. doi: 10.1128/aem.56.9.2698-2703.1990. PMID: 16348278; PMCID: PMC184830.
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Appl Environ Microbiol. 1990 Sep;56(9):2698-703. doi: 10.1128/aem.56.9.2698-2703.1990.

Adhesion of cellulolytic ruminal bacteria to barley straw.

Applied and environmental microbiology

S Bhat, R J Wallace, E R Orskov

Affiliations

  1. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, United Kingdom.

PMID: 16348278 PMCID: PMC184830 DOI: 10.1128/aem.56.9.2698-2703.1990

Abstract

Adhesion of the cellulolytic ruminal bacteria Ruminococcus flavefaciens and Fibrobacter succinogenes to barley straw was measured by incubating bacterial suspensions with hammer-milled straw for 30 min, filtering the mixtures through sintered glass filters, and measuring the optical densities of the filtrates. Maximum adhesion of both species occurred at pH 6.0 and during mid- to late-exponential phase. Adhesion was saturable at 33 and 23 mg (dry weight) g of straw for R. flavefaciens and F. succinogenes, respectively. Methyl cellulose and carboxymethyl cellulose inhibited adhesion by 24 to 33%. Competition between species was determined by measuring characteristic cell-associated enzyme activities in filtrates of mixtures incubated with straw; p-nitrophenyl-beta-d-lactopyranoside hydrolysis was used as a marker for F. succinogenes, while either beta-xylosidase or carboxymethyl cellulase was used for R. flavefaciens, depending on the other species present. R. flavefaciens had no influence on F. succinogenes adhesion, and F. succinogenes had only a minor (<20%) effect on R. flavefaciens adhesion. The noncellulolytic ruminal bacteria Bacteroides ruminicola and Selenomonas ruminantium had no influence on adhesion of either cellulolytic species, although these organisms also adhered to the straw. We concluded that R. flavefaciens and F. succinogenes have separate, specific adhesion sites on barley straw that are not obscured by competition with non-cellulolytic species.

References

  1. J Bacteriol. 1975 Apr;122(1):278-87 - PubMed
  2. Appl Environ Microbiol. 1978 Jun;35(6):1166-73 - PubMed
  3. Infect Immun. 1976 Oct;14(4):1109-12 - PubMed
  4. Appl Environ Microbiol. 1989 Dec;55(12):3039-44 - PubMed
  5. Appl Environ Microbiol. 1989 Aug;55(8):2089-91 - PubMed
  6. Br J Nutr. 1987 May;57(3):407-15 - PubMed
  7. Appl Environ Microbiol. 1988 Jun;54(6):1634-6 - PubMed
  8. Can J Microbiol. 1987 Mar;33(3):267-72 - PubMed
  9. Folia Microbiol (Praha). 1983;28(2):130-3 - PubMed
  10. Can J Microbiol. 1983 May;29(5):504-17 - PubMed
  11. Appl Environ Microbiol. 1977 Mar;33(3):497-502 - PubMed
  12. Appl Environ Microbiol. 1978 Jan;35(1):156-65 - PubMed
  13. Appl Environ Microbiol. 1978 Sep;36(3):513-22 - PubMed
  14. Appl Environ Microbiol. 1984 Sep;48(3):473-6 - PubMed
  15. Appl Environ Microbiol. 1988 Apr;54(4):890-7 - PubMed

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