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Doyle CJ, Hirst ML, Cory JS, et al. Risk Assessment Studies: Detailed Host Range Testing of Wild-Type Cabbage Moth, Mamestra brassicae (Lepidoptera: Noctuidae), Nuclear Polyhedrosis Virus. Appl Environ Microbiol. 1990;56(9):2704-10doi: 10.1128/aem.56.9.2704-2710.1990.
Doyle, C. J., Hirst, M. L., Cory, J. S., & Entwistle, P. F. (1990). Risk Assessment Studies: Detailed Host Range Testing of Wild-Type Cabbage Moth, Mamestra brassicae (Lepidoptera: Noctuidae), Nuclear Polyhedrosis Virus. Applied and environmental microbiology, 56(9), 2704-10. https://doi.org/10.1128/aem.56.9.2704-2710.1990
Doyle, C J, et al. "Risk Assessment Studies: Detailed Host Range Testing of Wild-Type Cabbage Moth, Mamestra brassicae (Lepidoptera: Noctuidae), Nuclear Polyhedrosis Virus." Applied and environmental microbiology vol. 56,9 (1990): 2704-10. doi: https://doi.org/10.1128/aem.56.9.2704-2710.1990
Doyle CJ, Hirst ML, Cory JS, Entwistle PF. Risk Assessment Studies: Detailed Host Range Testing of Wild-Type Cabbage Moth, Mamestra brassicae (Lepidoptera: Noctuidae), Nuclear Polyhedrosis Virus. Appl Environ Microbiol. 1990 Sep;56(9):2704-10. doi: 10.1128/aem.56.9.2704-2710.1990. PMID: 16348279; PMCID: PMC184831.
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Appl Environ Microbiol. 1990 Sep;56(9):2704-10. doi: 10.1128/aem.56.9.2704-2710.1990.

Risk Assessment Studies: Detailed Host Range Testing of Wild-Type Cabbage Moth, Mamestra brassicae (Lepidoptera: Noctuidae), Nuclear Polyhedrosis Virus.

Applied and environmental microbiology

C J Doyle, M L Hirst, J S Cory, P F Entwistle

Affiliations

  1. Natural Environment Research Council, Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, England.

PMID: 16348279 PMCID: PMC184831 DOI: 10.1128/aem.56.9.2704-2710.1990

Abstract

The host range of a multiply enveloped nuclear polyhedrosis virus (NPV) (Baculoviridae) isolated from the cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae), was determined by challenging a wide range of insect species with high (10 polyhedral inclusion bodies) and low (10 polyhedral inclusion bodies) doses of the virus. The identity of the progeny virus was confirmed by dot blotting. Analysis of 50% lethal dose was carried out on selected species, and the progeny virus was identified by using restriction enzyme analysis and Southern blotting. Other than the Lepidoptera, none of the species tested was susceptible to M. brassicae NPV. Within the Lepidoptera, M. brassicae NPV was infective to members of four families (Noctuidae, Geometridae, Yponomeutidae, and Nymphalidae). Of 66 lepidopterous species tested, M. brassicae NPV was cross-infective to 32 of them; however, 91% of the susceptible species were in the Noctuidae. The relevance of host range data in risk assessment studies is discussed.

References

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