Display options
Cite
Wachenheim DE, Patterson JA. Anaerobic Production of Extracellular Polysaccharide by Butyrivibrio fibrisolvens nyx. Appl Environ Microbiol. 1992;58(1):385-91doi: 10.1128/aem.58.1.385-391.1992.
Wachenheim, D. E., & Patterson, J. A. (1992). Anaerobic Production of Extracellular Polysaccharide by Butyrivibrio fibrisolvens nyx. Applied and environmental microbiology, 58(1), 385-91. https://doi.org/10.1128/aem.58.1.385-391.1992
Wachenheim, D E, and Patterson, J A. "Anaerobic Production of Extracellular Polysaccharide by Butyrivibrio fibrisolvens nyx." Applied and environmental microbiology vol. 58,1 (1992): 385-91. doi: https://doi.org/10.1128/aem.58.1.385-391.1992
Wachenheim DE, Patterson JA. Anaerobic Production of Extracellular Polysaccharide by Butyrivibrio fibrisolvens nyx. Appl Environ Microbiol. 1992 Jan;58(1):385-91. doi: 10.1128/aem.58.1.385-391.1992. PMID: 16348636; PMCID: PMC195219.
Copy Download .nbib Format: NLM
Share it on

Appl Environ Microbiol. 1992 Jan;58(1):385-91. doi: 10.1128/aem.58.1.385-391.1992.

Anaerobic Production of Extracellular Polysaccharide by Butyrivibrio fibrisolvens nyx.

Applied and environmental microbiology

D E Wachenheim, J A Patterson

Affiliations

  1. Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907.

PMID: 16348636 PMCID: PMC195219 DOI: 10.1128/aem.58.1.385-391.1992

Abstract

Anaerobic production of extracellular polysaccharide (EP) was examined, using a previously uncharacterized, obligately anaerobic rumen isolate, Butyrivibrio fibrisolvens nyx, which produced an EP that was rheologically similar to xanthan gum. The main objectives were to determine the nutritional requirements and conditions which promoted EP production by strain nyx. Strain nyx was grown anaerobically in defined and semidefined media. In addition to carbohydrate and nitrogen sources, strain nyx required acetic acid, folic acid, biotin, and pyridoxamine. Strain nyx produced similar amounts of EP at 35 to 40 degrees C. Conditions that improved growth usually improved EP production. Of the carbohydrates tested, glucose supported the fastest growth and most EP production, followed by sucrose, xylose, and lactose. Strain nyx utilized ammonium sulfate, urea, or vitamin-free casein hydrolysate as nitrogen sources for growth and EP production. At 2 and 20 g/liter, respectively, ammonium sulfate and vitamin-free casein hydrolysate supported about the same rates of growth and EP production. EP was not produced in the lag or stationary phases, and EP production was exponential during exponential cell growth. Based on the results of this work, anaerobic EP production with B. fibrisolvens nyx could reduce energy costs for industrial EP production compared with the cost of aerated systems. Finally, this work demonstrated that, under appropriate growth conditions, a gastrointestinal tract (ruminal) microorganism produced high levels of EP.

References

  1. Appl Environ Microbiol. 1988 Apr;54(4):878-83 - PubMed
  2. J Bacteriol. 1964 Sep;88:640-5 - PubMed
  3. Am J Clin Nutr. 1972 Dec;25(12):1324-8 - PubMed
  4. Appl Environ Microbiol. 1980 Apr;39(4):709-19 - PubMed
  5. Appl Environ Microbiol. 1982 Aug;44(2):499-501 - PubMed
  6. J Gen Microbiol. 1977 Sep;102(1):13-21 - PubMed
  7. J Bacteriol. 1978 May;134(2):418-22 - PubMed
  8. J Gen Microbiol. 1953 Oct;9(2):174-89 - PubMed
  9. Appl Environ Microbiol. 1979 Jun;37(6):1186-92 - PubMed
  10. Appl Environ Microbiol. 1982 Nov;44(5):1231-7 - PubMed
  11. Appl Environ Microbiol. 1986 Jun;51(6):1224-9 - PubMed
  12. Appl Environ Microbiol. 1988 Oct;54(10):2381-6 - PubMed
  13. Appl Environ Microbiol. 1988 Oct;54(10):2411-4 - PubMed
  14. Sci Am. 1978 Jan;238(1):86-95 - PubMed
  15. Appl Environ Microbiol. 1991 Jul;57(7):2016-20 - PubMed
  16. Appl Environ Microbiol. 1987 Dec;53(12):2849-53 - PubMed
  17. J Dairy Sci. 1980 Aug;63(8):1248-63 - PubMed
  18. Adv Carbohydr Chem Biochem. 1979;36:265-313 - PubMed
  19. Appl Environ Microbiol. 1986 Jul;52(1):51-8 - PubMed

Publication Types