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Fox A, Rosario RM, Larsson L. Monitoring of bacterial sugars and hydroxy Fatty acids in dust from air conditioners by gas chromatography-mass spectrometry. Appl Environ Microbiol. 1993;59(12):4354-60doi: 10.1128/aem.59.12.4354-4360.1993.
Fox, A., Rosario, R. M., & Larsson, L. (1993). Monitoring of bacterial sugars and hydroxy Fatty acids in dust from air conditioners by gas chromatography-mass spectrometry. Applied and environmental microbiology, 59(12), 4354-60. https://doi.org/10.1128/aem.59.12.4354-4360.1993
Fox, A, et al. "Monitoring of bacterial sugars and hydroxy Fatty acids in dust from air conditioners by gas chromatography-mass spectrometry." Applied and environmental microbiology vol. 59,12 (1993): 4354-60. doi: https://doi.org/10.1128/aem.59.12.4354-4360.1993
Fox A, Rosario RM, Larsson L. Monitoring of bacterial sugars and hydroxy Fatty acids in dust from air conditioners by gas chromatography-mass spectrometry. Appl Environ Microbiol. 1993 Dec;59(12):4354-60. doi: 10.1128/aem.59.12.4354-4360.1993. PMID: 16349134; PMCID: PMC195911.
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Appl Environ Microbiol. 1993 Dec;59(12):4354-60. doi: 10.1128/aem.59.12.4354-4360.1993.

Monitoring of bacterial sugars and hydroxy Fatty acids in dust from air conditioners by gas chromatography-mass spectrometry.

Applied and environmental microbiology

A Fox, R M Rosario, L Larsson

Affiliations

  1. Department of Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina 29208, and Department of Medical Microbiology, University of Lund, 223 62 Lund, Sweden.

PMID: 16349134 PMCID: PMC195911 DOI: 10.1128/aem.59.12.4354-4360.1993

Abstract

Bacterial levels in dust collected from hospital air-conditioning filters were examined by chemical analysis (without prior culture). The dust was analyzed by gas chromatography-mass spectrometry after hydrolysis and derivatization. l-Glycero-d-mannoheptose and hydroxy fatty acids (3-OH 12:0 and 3-OH 14:0) (primarily found in lipopolysaccharide) and muramic acid (a chemical marker for bacterial peptidoglycan) were present at higher levels in dust collected from filters primarily contacting outdoor (as opposed to indoor) air. The ratio of l-glycero-d-mannoheptose to muramic acid in dust (compared with those of a group of gram-positive and gram-negative bacteria) suggested that both dust types contained appreciable numbers of gram-negative bacteria. There is potential for the chemical assessment of the microbial content of airborne dust.

References

  1. APMIS. 1990 Nov;98(11):957-68 - PubMed
  2. Infect Immun. 1987 Jun;55(6):1526-8 - PubMed
  3. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1673-7 - PubMed
  4. Appl Environ Microbiol. 1984 Dec;48(6):1189-96 - PubMed
  5. Ann Intern Med. 1984 Aug;101(2):157-63 - PubMed
  6. J Toxicol Environ Health. 1982 Feb;9(2):339-49 - PubMed
  7. Biken J. 1977 Mar;20(1):5-10 - PubMed
  8. J Med Microbiol. 1992 Aug;37(2):81-2 - PubMed
  9. J Hosp Infect. 1991 Jun;18 Suppl A:451-9 - PubMed
  10. Appl Environ Microbiol. 1990 May;56(5):1271-8 - PubMed
  11. J Chromatogr. 1988 Sep 23;431(1):1-15 - PubMed
  12. Am Ind Hyg Assoc J. 1988 Feb;49(2):81-8 - PubMed
  13. Am Ind Hyg Assoc J. 1987 Aug;48(8):745-51 - PubMed
  14. Am Ind Hyg Assoc J. 1984 Nov;45(11):760-6 - PubMed
  15. Am Ind Hyg Assoc J. 1983 Jul;44(7):537-41 - PubMed
  16. J Clin Microbiol. 1984 Mar;19(3):326-32 - PubMed
  17. Infect Immun. 1980 Aug;29(2):526-31 - PubMed
  18. Am Ind Hyg Assoc J. 1977 Oct;38(10):554-9 - PubMed
  19. Infect Immun. 1991 Mar;59(3):1202-5 - PubMed

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