Appl Microbiol. 1963 Nov;11(6):506-12. doi: 10.1128/am.11.6.506-512.1963.
Applied microbiology
D M Gallup, P Gerhardt
PMID: 16349641 PMCID: PMC1058040 DOI: 10.1128/am.11.6.506-512.1963
Cell-mass production of Serratia marcescens was studied in dialysis systems in which growth was managed in a fermentor remote from, but connected by conduits and pumps with, a nutrient reservoir. Dialysis was accomplished with membrane tubing in either the fermentor or the reservoir, or best with membrane sheet in a plate-and-frame dialyzer that was remote from but connected with both vessels. Growth trials with these systems demonstrated their ability to produce virtually unlimited population density in a liquid culture, viable counts in excess of 10 cells/ml and partial cell volume of 50% being attained. The system used for growth also may be used, after the growth cycle, to concentrate cells still further by osmotic dehydration with a hydrophilic colloid. The dialyzer-dialysis system that was evolved permits independent control of the component operations and is believed to be adaptable to any desired scale of size.
