Display options
Cite
Bonen L, Boer PH, Gray MW. The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. EMBO J. 1984;3(11):2531-6
Bonen, L., Boer, P. H., & Gray, M. W. (1984). The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. The EMBO journal, 3(11), 2531-6.
Bonen, L, et al. "The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize." The EMBO journal vol. 3,11 (1984): 2531-6.
Bonen L, Boer PH, Gray MW. The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. EMBO J. 1984 Nov;3(11):2531-6. PMID: 16453565; PMCID: PMC557724.
Copy Download .nbib Format: NLM
Share it on

EMBO J. 1984 Nov;3(11):2531-6.

The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize.

The EMBO journal

L Bonen, P H Boer, M W Gray

Affiliations

  1. Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada.

PMID: 16453565 PMCID: PMC557724

Abstract

We have determined the sequence of the wheat mitochondrial gene for cytochrome oxidase subunit II (COII) and find that its derived protein sequence differs from that of maize at only three amino acid positions. Unexpectedly, all three replacements are non-conservative ones. The wheat COII gene has a highly-conserved intron at the same position as in maize, but the wheat intron is 1.5 times longer because of an insert relative to its maize counterpart. Hybridization analysis of mitochondrial DNA from rye, pea, broad bean and cucumber indicates strong sequence conservation of COII coding sequences among all these higher plants. However, only rye and maize mitochondrial DNA show homology with wheat COII intron sequences and rye alone with intron-insert sequences. We find that a sequence identical to the region of the 5' exon corresponding to the transmembrane domain of the COII protein is present at a second genomic location in wheat mitochondria. These variations in COII gene structure and size, as well as the presence of repeated COII sequences, illustrate at the DNA sequence level, factors which contribute to higher plant mitochondrial DNA diversity and complexity.

References

  1. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 - PubMed
  2. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1946-50 - PubMed
  3. Nucleic Acids Res. 1980 Jan 25;8(2):319-35 - PubMed
  4. Cell. 1981 Sep;25(3):793-803 - PubMed
  5. Proc Natl Acad Sci U S A. 1982 May;79(10):3246-50 - PubMed
  6. Biochim Biophys Acta. 1983 Jul 15;726(2):135-48 - PubMed
  7. EMBO J. 1983;2(7):1049-54 - PubMed
  8. Nature. 1982 Oct 21;299(5885):698-702 - PubMed
  9. Nature. 1981 Apr 9;290(5806):457-65 - PubMed
  10. EMBO J. 1983;2(1):33-8 - PubMed
  11. EMBO J. 1984 Feb;3(2):297-302 - PubMed
  12. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1967-71 - PubMed
  13. Cell. 1978 Dec;15(4):1109-23 - PubMed
  14. Hoppe Seylers Z Physiol Chem. 1979 Apr;360(4):613-9 - PubMed
  15. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
  16. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
  17. Biochemistry. 1977 Oct 18;16(21):4743-51 - PubMed
  18. Science. 1974 Sep 6;185(4154):862-4 - PubMed
  19. J Mol Biol. 1983 Feb 15;164(1):35-58 - PubMed
  20. J Biol Chem. 1980 Dec 25;255(24):11927-41 - PubMed
  21. Cell. 1981 Nov;26(3 Pt 1):315-23 - PubMed
  22. Gene. 1982 Oct;19(3):259-68 - PubMed
  23. Genetics. 1984 Mar;106(3):479-99 - PubMed
  24. Nucleic Acids Res. 1982 Jul 10;10(13):3921-32 - PubMed
  25. EMBO J. 1983;2(12):2173-8 - PubMed
  26. Cell. 1980 Jul;20(3):579-95 - PubMed

Publication Types