Display options
Cite
Kotoujansky A, Diolez A, Boccara M, et al. Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes. EMBO J. 1985;4(3):781-5
Kotoujansky, A., Diolez, A., Boccara, M., Bertheau, Y., Andro, T., & Coleno, A. (1985). Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes. The EMBO journal, 4(3), 781-5.
Kotoujansky, A, et al. "Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes." The EMBO journal vol. 4,3 (1985): 781-5.
Kotoujansky A, Diolez A, Boccara M, Bertheau Y, Andro T, Coleno A. Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes. EMBO J. 1985 Mar;4(3):781-5. PMID: 16453606; PMCID: PMC554256.
Copy Download .nbib Format: NLM
Share it on

EMBO J. 1985 Mar;4(3):781-5.

Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes.

The EMBO journal

A Kotoujansky, A Diolez, M Boccara, Y Bertheau, T Andro, A Coleno

Affiliations

  1. Laboratoire de Pathologie Végétale, Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France.

PMID: 16453606 PMCID: PMC554256

Abstract

Erwinia chrysanthemi 3937 secretes four major pectate lyase isoenzymes (PL, EC 4.2.2.2) and one endocellulase (Cx, EC 3.2.1.4). A genomic library of this strain was constructed in the Lambda L47-1 vector, and screened for the presence of PL and Cx on pectate and caboxymethylcellulose agar. Among the seven Cx-positive phage clones, three were shown to encode an enzyme of the same mol. wt. as the one found in the culture supernatant of strain 3937. The 34 PL-positive phage clones were analyzed by electrofocusing and could, according to the PL they produced, be arranged in five classes. Phages from three classes produced three different single PL, named PLb, c and d. No common fragment was evidenced between the inserts of the phages of these three classes. This demonstrated that, in strain 3937, PLb, C, and d were encoded by three different genes called pelB, C, and D. Furthermore, our results suggest the existence of two additional genes encoding PLa and e. In addition, a pectin methylesterase gene was found closely linked to pelD.

References

  1. Methods Enzymol. 1979;68:299-309 - PubMed
  2. Annu Rev Microbiol. 1972;26:389-426 - PubMed
  3. J Bacteriol. 1982 Apr;150(1):122-31 - PubMed
  4. J Bacteriol. 1983 Jun;154(3):1489-92 - PubMed
  5. Anal Biochem. 1980 Sep 1;107(1):177-81 - PubMed
  6. Methods Enzymol. 1979;68:281-98 - PubMed
  7. Cell. 1976 Sep;9(1):91-9 - PubMed
  8. J Bacteriol. 1977 Dec;132(3):862-9 - PubMed
  9. Gene. 1980 Aug;10(3):249-59 - PubMed
  10. J Bacteriol. 1984 Dec;160(3):1199-203 - PubMed
  11. Anal Biochem. 1984 Jun;139(2):383-9 - PubMed
  12. J Bacteriol. 1980 Sep;143(3):1444-9 - PubMed
  13. J Biol Chem. 1963 May;238:1571-83 - PubMed

Publication Types