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Hoffman LM, Donaldson DD, Bookland R, et al. Synthesis and protein body deposition of maize 15-kd zein in transgenic tobacco seeds. EMBO J. 1987;6(11):3213-21
Hoffman, L. M., Donaldson, D. D., Bookland, R., Rashka, K., & Herman, E. M. (1987). Synthesis and protein body deposition of maize 15-kd zein in transgenic tobacco seeds. The EMBO journal, 6(11), 3213-21.
Hoffman, L M, et al. "Synthesis and protein body deposition of maize 15-kd zein in transgenic tobacco seeds." The EMBO journal vol. 6,11 (1987): 3213-21.
Hoffman LM, Donaldson DD, Bookland R, Rashka K, Herman EM. Synthesis and protein body deposition of maize 15-kd zein in transgenic tobacco seeds. EMBO J. 1987 Nov;6(11):3213-21. PMID: 16453803; PMCID: PMC553772.
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EMBO J. 1987 Nov;6(11):3213-21.

Synthesis and protein body deposition of maize 15-kd zein in transgenic tobacco seeds.

The EMBO journal

L M Hoffman, D D Donaldson, R Bookland, K Rashka, E M Herman

Affiliations

  1. Agrigenetics Advanced Science Company, 5649 East Buckeye Road, Madison, WI 53716.

PMID: 16453803 PMCID: PMC553772

Abstract

The maize 15-Kd zein structural gene was placed under the regulation of French bean beta-phaseolin gene flanking regions. Agrobacterium tumefaciens-mediated transformation was used to insert the chimeric phaseolin-zein gene into the tobacco genome. Transgenic plants synthesized zein in a tissue-specific manner during the latter half of seed development. Transcription of the chimeric gene was initiated in phaseolin-derived sequences, and was terminated within the phaseolin gene 3' flanking region. Both zein- and phaseolin-derived polyadenylation signals were used in the processing of zein RNA in transgenic plant seeds. Zein accumulation, though subject to an 80-fold variation among 19 plants tested, could reach as much as 1.6% of the total seed protein in several plants. In developing tobacco seeds, zein was correctly processed by the removal of a 20-amino-acid signal peptide. Electron microscope immunogold localization of the zein expressed in embryo and endosperm tissue indicates that the monocot protein accumulates in the crystalloid component of vacuolar protein bodies. The density of gold label over the protein bodies is several fold greater in the embryo than the endosperm. Zein is found in roots, hypocotyls and cotyledons of germinating transgenic tobacco seeds.

References

  1. Nucleic Acids Res. 1985 Sep 25;13(18):6483-98 - PubMed
  2. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
  3. Nature. 1985 Jun 27-Jul 3;315(6022):750-2 - PubMed
  4. Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 - PubMed
  5. EMBO J. 1985 Dec 1;4(12):3047-53 - PubMed
  6. EMBO J. 1984 Jul;3(7):1525-31 - PubMed
  7. Anal Biochem. 1984 Feb;137(1):182-7 - PubMed
  8. Proc Natl Acad Sci U S A. 1985 May;82(10):3320-4 - PubMed
  9. Plant Physiol. 1978 Aug;62(2):256-63 - PubMed
  10. Science. 1985 Mar 8;227(4691):1229-31 - PubMed
  11. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  12. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
  13. Anal Biochem. 1986 Oct;158(1):165-70 - PubMed
  14. Gene. 1986;41(1):47-57 - PubMed
  15. J Biol Chem. 1986 May 15;261(14):6279-84 - PubMed
  16. Nucleic Acids Res. 1986 Mar 11;14(5):2229-40 - PubMed
  17. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  18. Anal Biochem. 1981 Apr;112(2):195-203 - PubMed
  19. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4803-7 - PubMed
  20. Anal Biochem. 1983 Jul 15;132(2):462-7 - PubMed
  21. J Biol Chem. 1981 Mar 25;256(6):2597-600 - PubMed
  22. J Agric Food Chem. 1980 Nov-Dec;28(6):1334-6 - PubMed
  23. EMBO J. 1986 Oct;5(10):2419-25 - PubMed
  24. Plant Physiol. 1974 Mar;53(3):453-7 - PubMed
  25. Plant Physiol. 1985 Sep;79(1):65-71 - PubMed
  26. Genetics. 1986 Sep;114(1):303-13 - PubMed
  27. J Immunol Methods. 1986 Feb 27;87(1):7-11 - PubMed

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