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Kirwin PM, Meadows JW, Shackleton JB, et al. ATP-dependent import of a lumenal protein by isolated thylakoid vesicles. EMBO J. 1989;8(8):2251-5
Kirwin, P. M., Meadows, J. W., Shackleton, J. B., Musgrove, J. E., Elderfield, P. D., Mould, R., Hay, N. A., & Robinson, C. (1989). ATP-dependent import of a lumenal protein by isolated thylakoid vesicles. The EMBO journal, 8(8), 2251-5.
Kirwin, P M, et al. "ATP-dependent import of a lumenal protein by isolated thylakoid vesicles." The EMBO journal vol. 8,8 (1989): 2251-5.
Kirwin PM, Meadows JW, Shackleton JB, Musgrove JE, Elderfield PD, Mould R, Hay NA, Robinson C. ATP-dependent import of a lumenal protein by isolated thylakoid vesicles. EMBO J. 1989 Aug;8(8):2251-5. PMID: 16453897; PMCID: PMC401155.
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EMBO J. 1989 Aug;8(8):2251-5.

ATP-dependent import of a lumenal protein by isolated thylakoid vesicles.

The EMBO journal

P M Kirwin, J W Meadows, J B Shackleton, J E Musgrove, P D Elderfield, R Mould, N A Hay, C Robinson

Affiliations

  1. Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.

PMID: 16453897 PMCID: PMC401155

Abstract

The 33 kd protein of the photosynthetic oxygen-evolving complex is synthesized in the cytoplasm as a larger precursor and transported into the thylakoid lumen via a stromal intermediate form. In this report we describe a reconstituted system in which the later stages of this import pathway can be studied in isolation. We demonstrate import of the 33 kd protein, probably as the intermediate form, into isolated pea thylakoids by a mechanism which is stimulated by the addition of ATP. The imported protein is processed to the mature size and is resistant to digestion by proteases. The thylakoidal protein transport system is specific in that non-chloroplast proteins and precursors of stromal proteins are not imported.

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