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Bryan JN, Lewis MR, Henry CJ, et al. Development of a two-antibody model for the evaluation of copper-64 radioimmunotherapy. Vet Comp Oncol. 2004;2(2):82-90doi: 10.1111/j.1476-5810.2004.00041.x.
Bryan, J. N., Lewis, M. R., Henry, C. J., Owen, N. K., Zhang, J., Mohsin, H., Jia, F., Sivaguru, G., & Anderson, C. J. (2004). Development of a two-antibody model for the evaluation of copper-64 radioimmunotherapy. Veterinary and comparative oncology, 2(2), 82-90. https://doi.org/10.1111/j.1476-5810.2004.00041.x
Bryan, J N, et al. "Development of a two-antibody model for the evaluation of copper-64 radioimmunotherapy." Veterinary and comparative oncology vol. 2,2 (2004): 82-90. doi: https://doi.org/10.1111/j.1476-5810.2004.00041.x
Bryan JN, Lewis MR, Henry CJ, Owen NK, Zhang J, Mohsin H, Jia F, Sivaguru G, Anderson CJ. Development of a two-antibody model for the evaluation of copper-64 radioimmunotherapy. Vet Comp Oncol. 2004 Jun;2(2):82-90. doi: 10.1111/j.1476-5810.2004.00041.x. PMID: 19379188.
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Vet Comp Oncol. 2004 Jun;2(2):82-90. doi: 10.1111/j.1476-5810.2004.00041.x.

Development of a two-antibody model for the evaluation of copper-64 radioimmunotherapy.

Veterinary and comparative oncology

J N Bryan, M R Lewis, C J Henry, N K Owen, J Zhang, H Mohsin, F Jia, G Sivaguru, C J Anderson

Affiliations

  1. Department of Veterinary Medicine and Surgery, University of Missouri-Columbia, Columbia, MO 65211, USA. [email protected]

PMID: 19379188 DOI: 10.1111/j.1476-5810.2004.00041.x

Abstract

Copper-64 emits beta(+) and beta(-) particles suitable for positron emission tomography and radioimmunotherapy (RIT) of cancer. Copper-64-labelled antibodies have caused complete responses in laboratory animal RIT studies at far lower radiation doses than traditionally prescribed. The intracellular localization of copper radioisotopes may lead to cytotoxic effects by mechanisms beyond ionizing radiation damage. The purpose of this research was to develop a model using both internalizing and non-internalizing antibodies for direct comparison in future RIT studies using the same animal model of cancer. The monoclonal antibodies, cBR96 and cT84.66, were conjugated with N-hydroxysulfosuccinimidyl DOTA. All conjugates retained high immunoreactivity and labelled efficiently with (64)Cu with high specific activity and radiochemical purity. Twenty-four hour biodistributions determined in LS174T tumour-bearing nude mice demonstrated low organ and high tumour uptakes for both monoclonal antibodies. This model constitutes a promising system for elucidating whether internalization of (64)Cu is responsible for an enhanced tumour cytotoxicity in vivo.

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