Immun Ageing. 2009 Mar 28;6:3. doi: 10.1186/1742-4933-6-3.

Ageing exacerbates damage of systemic and salivary neutrophils from patients presenting Candida-related denture stomatitis.

Immunity & ageing : I & A

Thaís Helena Gasparoto, Narciso Almeida Vieira, Vinicius Carvalho Porto, Ana Paula Campanelli, Vanessa Soares Lara

PMID: 19327169 PMCID: PMC2669447 DOI: 10.1186/1742-4933-6-3

Abstract

BACKGROUND: Ageing leads to a decline in the function of the immune system, increasing the body's susceptibility to infections through the impairment of T-cells, macrophages, neutrophils and dendritic cells Denture stomatitis is a primary oral disease affecting elderly denture wearers. The major etiologic factor involved in this pathology is the infection by Candida albicans, an opportunistic pathogen that causes local and disseminated diseases in immunosuppressed humans. Neutrophils play a critical role in the immune response against C. albicans and are continually present in the salivary fluid and in the blood. The aim of this study was to determine ageing-related changes in salivary and blood neutrophils and their potential implications in Candida-related denture stomatitis.

RESULTS: Our results showed a lower number of neutrophils in the saliva from patients presenting Candida-related denture stomatitis in comparison to their matched controls. Furthermore, fewer neutrophils were isolated from the saliva of aged control individuals in comparison to matched younger subjects. CXCR1, CD62L and CD11b expression were significantly greater on systemic neutrophils from younger control individuals. Elderly individuals showed more apoptotic salivary neutrophils and lower GM-CSF levels than younger ones, regardless of the occurrence of Candida infection. On the other hand, CXCL-8 concentrations were higher in the saliva from elderly individuals. Besides, TNF-alpha was detected at elevated levels in the saliva from infected elderly subjects. Salivary neutrophils from elderly and young patients presented impaired phagocytic activity against C. albicans. However, just systemic neutrophils from elderly showed decreased phagocytosis when compared to the younger ones, regardless of the occurrence of infection. In addition, neutrophils from aged individuals and young patients presented low fungicidal activity.

CONCLUSION: The data suggests that the Candida related-denture stomatitis is associated to neutrophils function deficiency, and ageing drastically appears to alter important characteristics of such cells, facilitating the establishment of this infection.

References

1. J Immunol. 2008 Mar 15;180(6):4124-32 - PubMed
2. J Anat. 2006 May;208(5):609-19 - PubMed
3. Blood. 2008 Jan 15;111(2):878-84 - PubMed
4. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 Jan;95(1):51-9 - PubMed
5. Ann Neurol. 1994;35 Suppl:S35-7 - PubMed
6. Oral Microbiol Immunol. 1988 Mar;3(1):8-13 - PubMed
7. Blood. 2002 Sep 1;100(5):1860-8 - PubMed
8. J Infect Dis. 2001 Jan 15;183(2):277-285 - PubMed
9. Community Dent Oral Epidemiol. 1991 Jun;19(3):173-5 - PubMed
10. J Immunol. 2000 Apr 15;164(8):4286-91 - PubMed
11. J Periodontal Res. 1980 Sep;15(5):463-9 - PubMed
12. J Dent Assoc S Afr. 1986 Jun;41(6):313-6 - PubMed
13. FEMS Immunol Med Microbiol. 2004 Oct 1;42(2):219-24 - PubMed
14. Am J Med Sci. 1972 Jun;263(6):451-6 - PubMed
15. J Can Dent Assoc. 2007 Nov;73(9):823-8 - PubMed
16. Oral Microbiol Immunol. 2002 Oct;17(5):311-4 - PubMed
17. J Med Microbiol. 2002 Nov;51(11):929-936 - PubMed
18. J Dent Res. 1981 Mar;60(3):729-30 - PubMed
19. J Immunol. 2008 Jan 1;180(1):475-82 - PubMed
20. Br J Haematol. 2005 Jun;129(5):569-82 - PubMed
21. Immunol Cell Biol. 2004 Aug;82(4):415-20 - PubMed
22. Crit Rev Oral Biol Med. 1990;1(2):117-33 - PubMed
23. Med Mycol. 2003 Feb;41(1):7-14 - PubMed
24. Mech Ageing Dev. 2003 Apr;124(4):419-25 - PubMed
25. Microbiology (Reading). 2004 Sep;150(Pt 9):2807-2813 - PubMed
26. Clin Exp Immunol. 1977 Jan;27(1):152-8 - PubMed
27. Infect Immun. 1995 Mar;63(3):984-8 - PubMed
28. Mech Ageing Dev. 1998 May 15;102(2-3):199-209 - PubMed
29. Mycoses. 2002 Oct;45(8):253-8 - PubMed
30. J Leukoc Biol. 2000 Jan;67(1):40-5 - PubMed
31. J Am Dent Assoc. 2003 Oct;134 Spec No:34S-40S - PubMed
32. J Periodontal Res. 1969;4(2):160 - PubMed
33. Crit Rev Immunol. 2000;20(3):197-222 - PubMed
34. Med Oral Patol Oral Cir Bucal. 2008 May 01;13(5):E270-4 - PubMed
35. Circulation. 1998 Jan 6-13;97(1):91-8 - PubMed
36. Mech Ageing Dev. 1993 Oct 1;71(1-2):143-54 - PubMed
37. Annu Rev Biomed Eng. 2005;7:151-85 - PubMed
38. Mech Ageing Dev. 1997 Jun;96(1-3):15-34 - PubMed
39. Gerontology. 1998;44(4):192-7 - PubMed
40. J Biol Chem. 2004 Jun 25;279(26):26915-21 - PubMed
41. J Oral Pathol Med. 2003 Nov;32(10):571-5 - PubMed
42. Oral Microbiol Immunol. 2006 Aug;21(4):252-5 - PubMed
43. Blood. 1991 May 15;77(10):2259-65 - PubMed
44. Ann Acad Med Singap. 2004 Jul;33(4 Suppl):27-30 - PubMed
45. J Oral Pathol Med. 2005 Jul;34(6):340-6 - PubMed
46. Biochem Biophys Res Commun. 1996 Feb 15;219(2):405-11 - PubMed
47. Mech Ageing Dev. 1984 Oct 15;27(2):233-7 - PubMed
48. J Dent Res. 1982 May;61(5):636-9 - PubMed
49. J Oral Pathol Med. 2003 May;32(5):271-4 - PubMed
50. PLoS Pathog. 2008 Feb 8;4(2):e35 - PubMed
51. J Immunol. 2001 Oct 1;167(7):4017-25 - PubMed
52. J Periodontol. 1981 Aug;52(8):410-5 - PubMed
53. Acta Odontol Scand. 2008;66(6):368-73 - PubMed
54. J Leukoc Biol. 2004 Aug;76(2):291-9 - PubMed
55. Immunology. 2007 Apr;120(4):435-46 - PubMed

Publication Types

• Journal Article