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Tefft PM, Bone LW. Leucine Aminopeptidase in Eggs of the Soybean Cyst Nematode Heterodera glycines. J Nematol. 1985;17(3):270-4
Tefft, P. M., & Bone, L. W. (1985). Leucine Aminopeptidase in Eggs of the Soybean Cyst Nematode Heterodera glycines. Journal of nematology, 17(3), 270-4.
Tefft, P M, and Bone, L W. "Leucine Aminopeptidase in Eggs of the Soybean Cyst Nematode Heterodera glycines." Journal of nematology vol. 17,3 (1985): 270-4.
Tefft PM, Bone LW. Leucine Aminopeptidase in Eggs of the Soybean Cyst Nematode Heterodera glycines. J Nematol. 1985 Jul;17(3):270-4. PMID: 19294093; PMCID: PMC2618468.
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J Nematol. 1985 Jul;17(3):270-4.

Leucine Aminopeptidase in Eggs of the Soybean Cyst Nematode Heterodera glycines.

Journal of nematology

P M Tefft, L W Bone

PMID: 19294093 PMCID: PMC2618468

Abstract

Supernatant from a sonicated macerate of eggs of Heterodera glycines hydrolyzed L-leucine beta-naphthylamide and L-leucine 7-amido-4-methylcoumarin. Rate of substrate hydrolysis was influenced by pH and increased with the duration of incubation. A Michaelis-Menten constant of 0.15 mM was obtained. Rate of substrate hydrolysis was decreased by freezing egg supernatant for 26 days or heating above 60 C for 5 minutes. When egg supernatant was incubated with six different substrates, L-leucine beta-naphthylamide was hydrolyzed most readily and L-valine beta-naphthylamide the least readily. The rate of substrate hydrolysis by egg supernatant was not increased by pretreatment of eggs with 3 mM zinc chloride for up to 14 days.

Keywords: Glycine max; biochemistry; egg hatching; enzyme; nematode eggs; soybean

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