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Musil S, Matoušek T. On-line pre-reduction of pentavalent arsenicals by thioglycolic acid for speciation analysis by selective hydride generation-cryotrapping-atomic absorption spectrometry. Spectrochim Acta Part B At Spectrosc. 2008;63(6):685-691doi: 10.1016/j.sab.2008.04.006.
Musil, S., & Matoušek, T. (2008). On-line pre-reduction of pentavalent arsenicals by thioglycolic acid for speciation analysis by selective hydride generation-cryotrapping-atomic absorption spectrometry. Spectrochimica acta. Part B, Atomic spectroscopy, 63(6), 685-691. https://doi.org/10.1016/j.sab.2008.04.006
Musil, Stanislav, and Matoušek, Tomáš. "On-line pre-reduction of pentavalent arsenicals by thioglycolic acid for speciation analysis by selective hydride generation-cryotrapping-atomic absorption spectrometry." Spectrochimica acta. Part B, Atomic spectroscopy vol. 63,6 (2008): 685-691. doi: https://doi.org/10.1016/j.sab.2008.04.006
Musil S, Matoušek T. On-line pre-reduction of pentavalent arsenicals by thioglycolic acid for speciation analysis by selective hydride generation-cryotrapping-atomic absorption spectrometry. Spectrochim Acta Part B At Spectrosc. 2008 Jun;63(6):685-691. doi: 10.1016/j.sab.2008.04.006. PMID: 19492007; PMCID: PMC2597875.
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Spectrochim Acta Part B At Spectrosc. 2008 Jun;63(6):685-691. doi: 10.1016/j.sab.2008.04.006.

On-line pre-reduction of pentavalent arsenicals by thioglycolic acid for speciation analysis by selective hydride generation-cryotrapping-atomic absorption spectrometry.

Spectrochimica acta. Part B, Atomic spectroscopy

Stanislav Musil, Tomáš Matoušek

Affiliations

  1. Institute of Analytical Chemistry of the ASCR, v.v.i., Vídeòská 1083, 14220 Prague, Czech Republic.

PMID: 19492007 PMCID: PMC2597875 DOI: 10.1016/j.sab.2008.04.006

Abstract

An improvement of current method of selective hydride generation based on pre-reduction for differentiation of tri- and pentavalent arsenicals is described, applied for the oxidation state specific speciation analysis of inorganic, mono-, di- and trimethylated arsenicals with minimum sample pretreatment using atomic absorption spectrometry with the multiatomizer. The preconcentration and separation of arsine, methylarsine, dimethylarsine and trimethylarsine is then carried out by means of cryotrapping. Presented study shows that 2% (m/v) L-cysteine hydrochloride monohydrate (L-cys) currently used for off-line pre-reduction of pentavalent arsenicals can be substituted with 1% (m/v) thioglycolic acid (TGA). Much faster pre-reduction of pentavalent arsenicals at 25°C with equal sensitivities as in the case of L-cys has been achieved with TGA. A setup for on-line pre-reduction by TGA has been optimized, with the application of segmented flow analysis for suppression of axial dispersion in the pre-reduction coil. Standard calibrations measured with or without on-line pre-reduction indicate uniform and equal sensitivities for all As forms. The possibility of standardization by water standards of single species (e.g. iAs(III)) for quantification of all other As forms in urine is demonstrated in the recovery study. Limits of detection were 100 ng·l(-1) for iAs(III), 135 ng·l(-1) for iAs(V) and 30 to 50 ng·l(-1) for methylated arsenicals.

References

  1. Toxicol Lett. 2002 Jul 7;133(1):1-16 - PubMed
  2. Environ Health Perspect. 2005 Mar;113(3):250-4 - PubMed
  3. Anal Chem. 2007 Aug 15;79(16):6324-33 - PubMed
  4. Toxicol Appl Pharmacol. 2001 Aug 1;174(3):282-93 - PubMed
  5. Spectrochim Acta Part B At Spectrosc. 2008 Mar;63(3):396-406 - PubMed
  6. Talanta. 2005 Jul 15;67(1):169-74 - PubMed
  7. Toxicol Appl Pharmacol. 2005 Sep 1;207(2):147-59 - PubMed
  8. Environ Health Perspect. 2006 Nov;114(11):1790-6 - PubMed
  9. Science. 1973 Dec 21;182(4118):1247-9 - PubMed
  10. Talanta. 2000 May 5;51(6):1059-68 - PubMed
  11. Exp Biol Med (Maywood). 2007 Jan;232(1):3-13 - PubMed
  12. Talanta. 2003 Mar 1;59(3):525-34 - PubMed
  13. J Anal At Spectrom. 2008;23:342-351 - PubMed
  14. Environ Health Perspect. 2006 Apr;114(4):527-31 - PubMed
  15. Chemosphere. 2004 Feb;54(8):1199-206 - PubMed

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