Display options
Cite
Kurczy ME, Piehowski PD, Parry SA, et al. Which is more important in bioimaging SIMS experiments-The sample preparation or the nature of the projectile?. Appl Surf Sci. 2008;255(4):1298-1304doi: 10.1016/j.apsusc.2008.05.139.
Kurczy, M. E., Piehowski, P. D., Parry, S. A., Jiang, M., Chen, G., Ewing, A. G., & Winograd, N. (2008). Which is more important in bioimaging SIMS experiments-The sample preparation or the nature of the projectile?. Applied surface science, 255(4), 1298-1304. https://doi.org/10.1016/j.apsusc.2008.05.139
Kurczy, M E, et al. "Which is more important in bioimaging SIMS experiments-The sample preparation or the nature of the projectile?." Applied surface science vol. 255,4 (2008): 1298-1304. doi: https://doi.org/10.1016/j.apsusc.2008.05.139
Kurczy ME, Piehowski PD, Parry SA, Jiang M, Chen G, Ewing AG, Winograd N. Which is more important in bioimaging SIMS experiments-The sample preparation or the nature of the projectile?. Appl Surf Sci. 2008 Dec 15;255(4):1298-1304. doi: 10.1016/j.apsusc.2008.05.139. PMID: 19554199; PMCID: PMC2700758.
Copy Download .nbib Format: NLM
Share it on

Appl Surf Sci. 2008 Dec 15;255(4):1298-1304. doi: 10.1016/j.apsusc.2008.05.139.

Which is more important in bioimaging SIMS experiments-The sample preparation or the nature of the projectile?.

Applied surface science

M E Kurczy, P D Piehowski, S A Parry, M Jiang, G Chen, A G Ewing, Nicholas Winograd

Affiliations

  1. Department of Chemistry, Penn State University, 104 Chemistry Building, University Park, PA 16802, United States.

PMID: 19554199 PMCID: PMC2700758 DOI: 10.1016/j.apsusc.2008.05.139

Abstract

Sample preparation is central to acquiring meaningful molecule-specific images with SIMS, especially when submicron lateral resolution is involved. The issue is to maintain the distribution of target molecules while attempting to introduce biological cells or tissue into the high vacuum environment of the mass spectrometer. Here we compare freeze-drying, freeze-etching, freeze-fracture and trehalose vitrification as possible strategies for these experiments. The results show that the prospects for successful imaging experiments are greatly improved with all of these methods when using cluster ion bombardment, particularly C(60) (+) ions, not only due to increased sensitivity of this projectiles, but also since it removes contamination overlayers without insult to the underlying chemistry. The emergence of 3-dimensional imaging capabilities also suggests that sample preparation should not perturb the 3-dimensional morphology of the cell, a situation not generally possible during freeze-drying. Hence, sample preparation and projectile type are strongly coupled parameters for bioimaging with mass spectrometry.

References

  1. J Cyclic Nucleotide Res. 1978 Feb;4(1):15-26 - PubMed
  2. Rapid Commun Mass Spectrom. 1998;12(18):1246-52 - PubMed
  3. Nat Biotechnol. 2000 Feb;18(2):145-6 - PubMed
  4. Anal Chem. 2003 Jul 15;75(14):3429-34 - PubMed
  5. Anal Chem. 2007 Mar 15;79(6):2199-206 - PubMed
  6. Anal Chem. 2006 Jan 1;78(1):141-8 - PubMed
  7. Anal Chem. 2006 Mar 15;78(6):1827-31 - PubMed
  8. Biol Cell. 1992;74(1):31-42 - PubMed
  9. Langmuir. 2007 Jul 17;23(15):8035-41 - PubMed
  10. Anal Chem. 1997 Jul 1;69(13):2225-31 - PubMed
  11. Anal Chem. 2007 Aug 1;79(15):5529-39 - PubMed
  12. Anal Chem. 2005 Dec 15;77(24):7950-7 - PubMed
  13. Anal Chem. 2003 Aug 15;75(16):4087-94 - PubMed
  14. Science. 2004 Jul 2;305(5680):71-3 - PubMed
  15. Langmuir. 2008 Aug 5;24(15):7906-11 - PubMed
  16. Anal Chem. 2007 May 15;79(10):3554-60 - PubMed
  17. Anal Chem. 2007 May 15;79(10):3535-41 - PubMed
  18. Rapid Commun Mass Spectrom. 2006;20(8):1327-34 - PubMed

Publication Types

Grant support