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Böhler T, Mrosek V, Müller K, et al. Cross-clade recognition of HIV-1 CAp24 by CD4+ T cells in HIV-1-infected individuals in Burkina Faso and Germany. Open AIDS J. 2009;3:4-7doi: 10.2174/1874613600903010004.
Böhler, T., Mrosek, V., Müller, K., Schnitzler, P., Hartmann, M., Ouedraogo, T., Coulibaly, B., Sié, A., Bartonova, V., Tebit, D. M., & Kräusslich, H. G. (2009). Cross-clade recognition of HIV-1 CAp24 by CD4+ T cells in HIV-1-infected individuals in Burkina Faso and Germany. The open AIDS journal, 34-7. https://doi.org/10.2174/1874613600903010004
Böhler, Thomas, et al. "Cross-clade recognition of HIV-1 CAp24 by CD4+ T cells in HIV-1-infected individuals in Burkina Faso and Germany." The open AIDS journal vol. 3 (2009): 4-7. doi: https://doi.org/10.2174/1874613600903010004
Böhler T, Mrosek V, Müller K, Schnitzler P, Hartmann M, Ouedraogo T, Coulibaly B, Sié A, Bartonova V, Tebit DM, Kräusslich HG. Cross-clade recognition of HIV-1 CAp24 by CD4+ T cells in HIV-1-infected individuals in Burkina Faso and Germany. Open AIDS J. 2009 Jan 23;3:4-7. doi: 10.2174/1874613600903010004. PMID: 19554213; PMCID: PMC2701271.
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Open AIDS J. 2009 Jan 23;3:4-7. doi: 10.2174/1874613600903010004.

Cross-clade recognition of HIV-1 CAp24 by CD4+ T cells in HIV-1-infected individuals in Burkina Faso and Germany.

The open AIDS journal

Thomas Böhler, Vanessa Mrosek, Kerstin Müller, Paul Schnitzler, Martin Hartmann, Thierry Ouedraogo, Boubacar Coulibaly, Ali Sié, Vanda Bartonova, Denis M Tebit, Hans-Georg Kräusslich

Affiliations

  1. Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany. [email protected]

PMID: 19554213 PMCID: PMC2701271 DOI: 10.2174/1874613600903010004

Abstract

The presence of antigen-specific cellular immune responses may be an indicator of long-term asymptomatic HIV-1-disease. The detection of cellular immune responses to infection with different subtypes of HIV-1 may be hampered by genetic differences of immunodominant antigens such as the capsid protein CAp24. In Nouna, Burkina Faso, HIV-1 circulating recombinant forms CRF02_AG and CRF06_cpx are the 2 major strains detectable in HIV-1-infected individuals, while subtype B strains prevail in Europe and North America. Amino acid sequences of CAp24 were assessed in blood samples from 10 HIV-1-infected patients in Nouna, Burkina Faso. Production of interferon-gamma (IFN-gamma) in peripheral blood CD4(+) lymphocytes in response to recombinant HIV-1 proteins derived from clade B (including CAp24(NL4-3)) was measured using a modified flow-cytometry-based whole blood short term activation assay (FASTimmune, BDBiosciences). IFN-gamma production following stimulation with a whole length CAp24 protein derived from clade B (CAp24(NL4-3)) was additionally quantified in comparison to a CAp24 protein derived from CRF02_AG (CAp24(BD6-15)) in 16 HIV-1-infected patients in Heidelberg, Germany. Amino acid sequence identity of CAp24 obtained from patients in Nouna ranged between 86 and 89% when compared to the clade B CAp24(NL4-3) consensus sequence, between 90 and 95% when compared to the circulating recombinant form CRF06_CPX consensus sequence, and between 92 and 96% when compared to the CAp24(BD6-15) consensus sequence. Significant numbers of HIV-1-specific CD4(+) lymphocytes producing IFN-gamma were detected in 4 of 10 HIV-1-infected patients. In 7 of 16 patients in Heidelberg, recombinant CAp24(BD6-15) stimulated IFN-gamma-production in CD4(+) lymphocytes to a similar extent as the clade B-derived CAp24(NL4-3). Thus, antigen-specific CD4(+) lymphocytes from both West African and European patients infected with different strains of HIV-1 show relevant cross-clade recognition of HIV-1 CAp24 in a flow-cytometry-based whole blood short term activation assay.

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