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Marsh P, Burns D, Girkin J. Practical implementation of adaptive optics in multiphoton microscopy. Opt Express. 2003;11(10):1123-30doi: 10.1364/oe.11.001123.
Marsh, P., Burns, D., & Girkin, J. (2003). Practical implementation of adaptive optics in multiphoton microscopy. Optics express, 11(10), 1123-30. https://doi.org/10.1364/oe.11.001123
Marsh, P, et al. "Practical implementation of adaptive optics in multiphoton microscopy." Optics express vol. 11,10 (2003): 1123-30. doi: https://doi.org/10.1364/oe.11.001123
Marsh P, Burns D, Girkin J. Practical implementation of adaptive optics in multiphoton microscopy. Opt Express. 2003 May 19;11(10):1123-30. doi: 10.1364/oe.11.001123. PMID: 19465977.
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Opt Express. 2003 May 19;11(10):1123-30. doi: 10.1364/oe.11.001123.

Practical implementation of adaptive optics in multiphoton microscopy.

Optics express

P Marsh, D Burns, J Girkin

PMID: 19465977 DOI: 10.1364/oe.11.001123

Abstract

A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 microm with a resolution defined by a FWHM axial point-spread function of 1.25 microm.

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