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Lasker MV, Thai P, Besant PG, et al. Branched-chain 6-ketoacid dehydrogenase kinase: A mammalian enzyme with histidine kinase activity. J Biomol Tech. 2002;13(4):238-45
Lasker, M. V., Thai, P., Besant, P. G., Bui, C. D., Naidu, S., & Turck, C. W. (2002). Branched-chain 6-ketoacid dehydrogenase kinase: A mammalian enzyme with histidine kinase activity. Journal of biomolecular techniques : JBT, 13(4), 238-45.
Lasker, Michael V, et al. "Branched-chain 6-ketoacid dehydrogenase kinase: A mammalian enzyme with histidine kinase activity." Journal of biomolecular techniques : JBT vol. 13,4 (2002): 238-45.
Lasker MV, Thai P, Besant PG, Bui CD, Naidu S, Turck CW. Branched-chain 6-ketoacid dehydrogenase kinase: A mammalian enzyme with histidine kinase activity. J Biomol Tech. 2002 Dec;13(4):238-45. PMID: 19498989; PMCID: PMC2279873.
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J Biomol Tech. 2002 Dec;13(4):238-45.

Branched-chain 6-ketoacid dehydrogenase kinase: A mammalian enzyme with histidine kinase activity.

Journal of biomolecular techniques : JBT

Michael V Lasker, Philip Thai, Paul G Besant, Cuong D Bui, Srinivas Naidu, Christoph W Turck

Affiliations

  1. Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, USA.

PMID: 19498989 PMCID: PMC2279873

Abstract

Whereas phosphoesters of serine, threonine, and tyrosine are present in great abundance in mammalian cells, only limited information is available for other amino acids modified by a phosphate group. Phosphohistidine in proteins has been discovered in mammalian cells, but no enzyme with histidine kinase activity has been reported to date. The present study demonstrates for the first time the histidine kinase activity of a mammalian protein. Branched-chain alpha-ketoacid dehydrogenase kinase, a mitochondrial enzyme, has high sequence similarity with histidine kinases from lower organisms but has been classified as a serine/threonine kinase. Our studies indicate that in addition to a serine this enzyme also autophosphorylates a histidine residue. This finding suggests that histidine kinases are not restricted to lower organisms.

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