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de Vrije T, Bakker RR, Budde MA, et al. Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana. Biotechnol Biofuels. 2009;2(1):12doi: 10.1186/1754-6834-2-12.
de Vrije, T., Bakker, R. R., Budde, M. A., Lai, M. H., Mars, A. E., & Claassen, P. A. (2009). Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana. Biotechnology for biofuels, 2(1), 12. https://doi.org/10.1186/1754-6834-2-12
de Vrije, Truus, et al. "Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana." Biotechnology for biofuels vol. 2,1 (2009): 12. doi: https://doi.org/10.1186/1754-6834-2-12
de Vrije T, Bakker RR, Budde MA, Lai MH, Mars AE, Claassen PA. Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana. Biotechnol Biofuels. 2009 Jun 17;2(1):12. doi: 10.1186/1754-6834-2-12. PMID: 19534765; PMCID: PMC2701949.
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Biotechnol Biofuels. 2009 Jun 17;2(1):12. doi: 10.1186/1754-6834-2-12.

Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana.

Biotechnology for biofuels

Truus de Vrije, Robert R Bakker, Miriam Aw Budde, Man H Lai, Astrid E Mars, Pieternel Am Claassen

Affiliations

  1. Agrotechnology and Food Sciences Group, Wageningen University and Research Centre, PO Box 17, 6700 AA Wageningen, the Netherlands. [email protected]

PMID: 19534765 PMCID: PMC2701949 DOI: 10.1186/1754-6834-2-12

Abstract

BACKGROUND: The production of hydrogen from biomass by fermentation is one of the routes that can contribute to a future sustainable hydrogen economy. Lignocellulosic biomass is an attractive feedstock because of its abundance, low production costs and high polysaccharide content.

RESULTS: Batch cultures of Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana produced hydrogen, carbon dioxide and acetic acid as the main products from soluble saccharides in Miscanthus hydrolysate. The presence of fermentation inhibitors, such as furfural and 5-hydroxylmethyl furfural, in this lignocellulosic hydrolysate was avoided by the mild alkaline-pretreatment conditions at a low temperature of 75 degrees C. Both microorganisms simultaneously and completely utilized all pentoses, hexoses and oligomeric saccharides up to a total concentration of 17 g l-1 in pH-controlled batch cultures. T. neapolitana showed a preference for glucose over xylose, which are the main sugars in the hydrolysate. Hydrogen yields of 2.9 to 3.4 mol H2 per mol of hexose, corresponding to 74 to 85% of the theoretical yield, were obtained in these batch fermentations. The yields were higher with cultures of C. saccharolyticus compared to T. neapolitana. In contrast, the rate of substrate consumption and hydrogen production was higher with T. neapolitana. At substrate concentrations exceeding 30 g l-1, sugar consumption was incomplete, and lower hydrogen yields of 2.0 to 2.4 mol per mol of consumed hexose were obtained.

CONCLUSION: Efficient hydrogen production in combination with simultaneous and complete utilization of all saccharides has been obtained during the growth of thermophilic bacteria on hydrolysate of the lignocellulosic feedstock Miscanthus. The use of thermophilic bacteria will therefore significantly contribute to the energy efficiency of a bioprocess for hydrogen production from biomass.

References

  1. Biotechnol Bioeng. 2006 Jan 5;93(1):56-63 - PubMed
  2. FEMS Microbiol Lett. 1994 Jul 15;120(3):263-6 - PubMed
  3. Bioresour Technol. 2005 Dec;96(18):1994-2006 - PubMed
  4. Nat Biotechnol. 2008 Feb;26(2):169-72 - PubMed
  5. Appl Biochem Biotechnol. 2004 Spring;113-116:497-508 - PubMed
  6. Biotechnol Bioeng. 2003 Feb 5;81(3):255-62 - PubMed
  7. Bioresour Technol. 2005 Apr;96(6):673-86 - PubMed
  8. Biotechnol Biofuels. 2008 Jun 11;1(1):12 - PubMed
  9. Appl Environ Microbiol. 2008 Nov;74(21):6720-9 - PubMed
  10. Bioresour Technol. 2006 Feb;97(3):500-5 - PubMed
  11. Bioresour Technol. 2005 Nov;96(17):1907-13 - PubMed
  12. Appl Biochem Biotechnol. 2002 Spring;98-100:327-40 - PubMed
  13. Appl Environ Microbiol. 1995 May;61(5):1867-75 - PubMed
  14. Biotechnol Bioeng. 1999 Oct 5;65(1):24-33 - PubMed
  15. Appl Microbiol Biotechnol. 2007 Apr;74(6):1358-67 - PubMed
  16. Water Sci Technol. 2005;52(1-2):21-9 - PubMed
  17. Biotechnol Bioeng. 2007 Aug 15;97(6):1460-9 - PubMed
  18. Biotechnol Bioeng. 1989 May;33(11):1495-9 - PubMed
  19. Bioprocess Biosyst Eng. 2009 Aug;32(5):603-6 - PubMed
  20. Appl Microbiol Biotechnol. 2008 Apr;78(6):939-45 - PubMed
  21. Curr Opin Biotechnol. 2008 Jun;19(3):210-7 - PubMed
  22. Ann N Y Acad Sci. 2008 Mar;1125:322-37 - PubMed

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