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Vermot J, Fraser SE, Liebling M. Fast fluorescence microscopy for imaging the dynamics of embryonic development. HFSP J. 2008;2(3):143-55doi: 10.2976/1.2907579.
Vermot, J., Fraser, S. E., & Liebling, M. (2008). Fast fluorescence microscopy for imaging the dynamics of embryonic development. HFSP journal, 2(3), 143-55. https://doi.org/10.2976/1.2907579
Vermot, Julien, et al. "Fast fluorescence microscopy for imaging the dynamics of embryonic development." HFSP journal vol. 2,3 (2008): 143-55. doi: https://doi.org/10.2976/1.2907579
Vermot J, Fraser SE, Liebling M. Fast fluorescence microscopy for imaging the dynamics of embryonic development. HFSP J. 2008 Jun;2(3):143-55. doi: 10.2976/1.2907579. Epub 2008 May 13. PMID: 19404468; PMCID: PMC2645566.
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HFSP J. 2008 Jun;2(3):143-55. doi: 10.2976/1.2907579. Epub 2008 May 13.

Fast fluorescence microscopy for imaging the dynamics of embryonic development.

HFSP journal

Julien Vermot, Scott E Fraser, Michael Liebling

PMID: 19404468 PMCID: PMC2645566 DOI: 10.2976/1.2907579

Abstract

Live imaging has gained a pivotal role in developmental biology since it increasingly allows real-time observation of cell behavior in intact organisms. Microscopes that can capture the dynamics of ever-faster biological events, fluorescent markers optimal for in vivo imaging, and, finally, adapted reconstruction and analysis programs to complete data flow all contribute to this success. Focusing on temporal resolution, we discuss how fast imaging can be achieved with minimal prejudice to spatial resolution, photon count, or to reliably and automatically analyze images. In particular, we show how integrated approaches to imaging that combine bright fluorescent probes, fast microscopes, and custom post-processing techniques can address the kinetics of biological systems at multiple scales. Finally, we discuss remaining challenges and opportunities for further advances in this field.

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