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Zaki MS, Eid RA. Role of vitamin-E on rat liver-amiodarone: an ultrastructural study. Saudi J Gastroenterol. 2009;15(2):104-10doi: 10.4103/1319-3767.48967.
Zaki, M. S., & Eid, R. A. (2009). Role of vitamin-E on rat liver-amiodarone: an ultrastructural study. Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association, 15(2), 104-10. https://doi.org/10.4103/1319-3767.48967
Zaki, Mohamed Samir A, and Eid, Refaat A. "Role of vitamin-E on rat liver-amiodarone: an ultrastructural study." Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association vol. 15,2 (2009): 104-10. doi: https://doi.org/10.4103/1319-3767.48967
Zaki MS, Eid RA. Role of vitamin-E on rat liver-amiodarone: an ultrastructural study. Saudi J Gastroenterol. 2009 Apr;15(2):104-10. doi: 10.4103/1319-3767.48967. PMID: 19568574; PMCID: PMC2702968.
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Saudi J Gastroenterol. 2009 Apr;15(2):104-10. doi: 10.4103/1319-3767.48967.

Role of vitamin-E on rat liver-amiodarone: an ultrastructural study.

Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association

Mohamed Samir A Zaki, Refaat A Eid

Affiliations

  1. Department of Anatomy, Faculty of Medicine, King Khalid University, Abha, Saudi Arabia. [email protected]

PMID: 19568574 PMCID: PMC2702968 DOI: 10.4103/1319-3767.48967

Abstract

BACKGROUND/AIM: Amiodarone, a class III antiarrhythmic drug, has been found to be effective in the management of patients with life-threatening ventricular arrhythmias. The aim of this study was to test whether the co administration of vitamin-E with amiodarone can reduce amiodarone-induced liver damage.

MATERIALS AND METHODS: Twelve male albino rats were divided into three groups (ml vegetable oil/day by oral gavages daily for 2 weeks and were used as control group. The rats of the second group received 5.4 mg amiodarone/100 gm rat dissolved in vegetable oil daily by oral gavages for 2 weeks. In the third group, the rats received 5.4 mg amiodarone and 5 mg vitamin-E/100 gram rat dissolved in 2 ml vegetable oil by oral gavages daily for 2 weeks. Two weeks after treatment, the rats were sacrificed and liver specimens were immediately taken and processed for transmission electron microscopic examinations.

RESULTS: Sections from the rat liver receiving amiodarone examined by electron microscopy showed disrupted hepatocytes with increased vacuolations. Degenerated organelles and disrupted nuclei were observed. The microvilli of bile canaliculi were disrupted and the hepatocytes showed increased lipid contents. Both endothelial cells and Kupffer cells were damaged. Phospholipids inside the mitochondria showed a loss of cristae. Sections from the liver of rats received amiodarone and vitamin-E showed lesser effects, especially in depositions of phospholipids in the mitochondria and the whole organelles and the nucleus showed minor damage in comparison to the previous group.

CONCLUSION: Milder hepatotoxic effects are seen in rats administered amiodarone and vitamin E simultaneously suggesting that vitamin-E may play a role in amelioration of the effects of amiodarone.

Keywords: Vitamin-E; amiodarone; rat liver; ultrastructural study

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