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Wang NY, Zhang D, Zhao W, et al. Clinical application of an enzyme-linked immunosorbent assay detecting hepatoma-specific gamma-glutamyltransferase. Hepatol Res. 2009;39(10):979-87doi: 10.1111/j.1872-034X.2009.00538.x.
Wang, N. Y., Zhang, D., Zhao, W., Fang, G. X., Shi, Y. L., & Duan, M. H. (2009). Clinical application of an enzyme-linked immunosorbent assay detecting hepatoma-specific gamma-glutamyltransferase. Hepatology research : the official journal of the Japan Society of Hepatology, 39(10), 979-87. https://doi.org/10.1111/j.1872-034X.2009.00538.x
Wang, Nian-Yue, et al. "Clinical application of an enzyme-linked immunosorbent assay detecting hepatoma-specific gamma-glutamyltransferase." Hepatology research : the official journal of the Japan Society of Hepatology vol. 39,10 (2009): 979-87. doi: https://doi.org/10.1111/j.1872-034X.2009.00538.x
Wang NY, Zhang D, Zhao W, Fang GX, Shi YL, Duan MH. Clinical application of an enzyme-linked immunosorbent assay detecting hepatoma-specific gamma-glutamyltransferase. Hepatol Res. 2009 Oct;39(10):979-87. doi: 10.1111/j.1872-034X.2009.00538.x. Epub 2009 Jul 13. PMID: 19624768.
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Hepatol Res. 2009 Oct;39(10):979-87. doi: 10.1111/j.1872-034X.2009.00538.x. Epub 2009 Jul 13.

Clinical application of an enzyme-linked immunosorbent assay detecting hepatoma-specific gamma-glutamyltransferase.

Hepatology research : the official journal of the Japan Society of Hepatology

Nian-Yue Wang, Dai Zhang, Wei Zhao, Guo-Xiang Fang, Yu-Ling Shi, Mu-Hao Duan

Affiliations

  1. Department of Clinical Laboratory, Nanjing Second Hospital Affiliated to Medical College, Southeast University, Nanjing, China.

PMID: 19624768 DOI: 10.1111/j.1872-034X.2009.00538.x

Abstract

AIM: To develop a sandwich enzyme-linked immunosorbent assay (ELISA) measuring hepatoma-specific datura stramonium agglutinin-tightly bounding gamma-glutamyltransferase (DSA-GGT) and evaluate its clinical application for hepatocellular carcinoma (HCC) diagnosis.

METHODS: Serum DSA-GGT concentrations were measured with the sandwich ELISA system in 96 patients with HCC, 240 patients with chronic liver diseases and 119 healthy subjects. The diagnostic performance of DSA-GGT for HCC was assessed using receiver operating characteristic (ROC) curves. The diagnostic accuracy of DSA-GGT was compared with serum alpha-fetoprotein (AFP).

RESULTS: The area under the ROC curve of DSA-GGT in discriminating patients with HCC from non-HCC was 0.865 (95% confidence interval: 0.818-0.915, P < 0.001). Serum DSA-GGT was positive in 67 out of 96 patients with HCC and 23 out of 240 patients with non-HCC diseases. The sensitivity and specificity of DSA-GGT and AFP for the diagnosis of HCC were 69.8% and 90.5%, and 72.9% and 89.1%, respectively. A higher sensitivity (93.8%) in the identification of HCC was observed by combining DSA-GGT and AFP.

CONCLUSION: The sandwich ELISA system showed good reliability and reproducibility, and using the measurement, we found that serum DSA-GGT was a valuable marker of HCC, as a usable complementary to AFP. The sensitivity for identifying HCC could be significantly improved by combining DSA-GGT and AFP, and the combination could be used in large-scale screening for HCC in susceptible individuals.

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