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Romanowicz L, Jaworski S, Galewska Z, et al. Separation and Determination of Fatty Acids from Lipid Fractions by High-Performance Liquid Chromatography: Cholesterol Esters of Umbilical Cord Arteries. Toxicol Mech Methods. 2008;18(6):509-513doi: 10.1080/15376510701623912.
Romanowicz, L., Jaworski, S., Galewska, Z., & Gogiel, T. (2008). Separation and Determination of Fatty Acids from Lipid Fractions by High-Performance Liquid Chromatography: Cholesterol Esters of Umbilical Cord Arteries. Toxicology mechanisms and methods, 18(6), 509-513. https://doi.org/10.1080/15376510701623912
Romanowicz, Lech, et al. "Separation and Determination of Fatty Acids from Lipid Fractions by High-Performance Liquid Chromatography: Cholesterol Esters of Umbilical Cord Arteries." Toxicology mechanisms and methods vol. 18,6 (2008): 509-513. doi: https://doi.org/10.1080/15376510701623912
Romanowicz L, Jaworski S, Galewska Z, Gogiel T. Separation and Determination of Fatty Acids from Lipid Fractions by High-Performance Liquid Chromatography: Cholesterol Esters of Umbilical Cord Arteries. Toxicol Mech Methods. 2008 Jul;18(6):509-513. doi: 10.1080/15376510701623912. Epub 2008 Jun 23. PMID: 19696943; PMCID: PMC2728760.
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Toxicol Mech Methods. 2008 Jul;18(6):509-513. doi: 10.1080/15376510701623912. Epub 2008 Jun 23.

Separation and Determination of Fatty Acids from Lipid Fractions by High-Performance Liquid Chromatography: Cholesterol Esters of Umbilical Cord Arteries.

Toxicology mechanisms and methods

Lech Romanowicz, Stefan Jaworski, Zofia Galewska, Tomasz Gogiel

Affiliations

  1. Department of Medical Biochemistry and Department of Gynecology, Medical University of Bia?ystok, Bia?ystok, Poland.

PMID: 19696943 PMCID: PMC2728760 DOI: 10.1080/15376510701623912

Abstract

Preeclampsia is accompanied by an extensive remodeling of the extracellular matrix of umbilical cord. It is associated with an increase in collagen content in the umbilical cord artery. Furthermore, preeclampsia distinctly reduces proteolytic and gelatinolytic activity, especially after activation with various agents.We decided to develop a method for separation and determination of fatty acids from different tissues by high-performance liquid chromatography. That method allowed us to determine cholesteryl ester composition and content in umbilical cord arteries. Studies were performed on the umbilical cord arteries taken from 10 newborns delivered by healthy mothers and 10 newborns delivered by mothers with preeclampsia. Cholesteryl esters were isolated by thin layer chromatography. Fatty acids were liberated by basic hydrolysis and analyzed by HPLC of their p-bromophenacyl derivatives using detection at 254 nm. It was found that saturated fatty acids were the main group of fatty acids incorporated to cholesteryl esters in all control and preeclamptic umbilical cord arteries. Preeclampsia caused a significant increase in cholesteryl ester content in the umbilical cord arteries. An increase of neutral lipid content in vessel walls of newborns delivered by mothers with preeclampsia may be one of the factors that evoke the initiation of hypertension in utero and its amplification throughout childhood and adult life. The described method reduces time and cost consumption and allows us to determine almost all fatty acids forming cholesteryl esters contained in the tissue sample.

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