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Wenger J, Rigneault H, Dintinger J, et al. Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture. J Biol Phys. 2006;32(1):SN1-4doi: 10.1007/s10867-006-2909-x.
Wenger, J., Rigneault, H., Dintinger, J., Marguet, D., & Lenne, P. F. (2006). Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture. Journal of biological physics, 32(1), SN1-4. https://doi.org/10.1007/s10867-006-2909-x
Wenger, Jérôme, et al. "Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture." Journal of biological physics vol. 32,1 (2006): SN1-4. doi: https://doi.org/10.1007/s10867-006-2909-x
Wenger J, Rigneault H, Dintinger J, Marguet D, Lenne PF. Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture. J Biol Phys. 2006 Jan;32(1):SN1-4. doi: 10.1007/s10867-006-2909-x. PMID: 19669430; PMCID: PMC3022499.
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J Biol Phys. 2006 Jan;32(1):SN1-4. doi: 10.1007/s10867-006-2909-x.

Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture.

Journal of biological physics

Jérôme Wenger, Hervé Rigneault, José Dintinger, Didier Marguet, Pierre-François Lenne

Affiliations

  1. Institut Fresnel, Université Paul Cézanne Aix-Marseille III, CNRS UMR 6133, Domaine Universitaire de Saint Jérôme, 13397 Marseille, Cedex 20, France.

PMID: 19669430 PMCID: PMC3022499 DOI: 10.1007/s10867-006-2909-x

Abstract

We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of beta-Bodipy-FL-C(5)-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This spatial resolution improvement comes together with an enhancement of the detected fluorescence per molecule as compared to an open sample, with a significant increase up to 3.5 times.

References

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