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Litinsky I, Golan I, Yaron M, et al. Simvastatin induces apoptosis of fibroblast-like synoviocytes. Open Rheumatol J. 2009;3:35-40doi: 10.2174/1874312900903010035.
Litinsky, I., Golan, I., Yaron, M., Yaron, I., Caspi, D., & Elkayam, O. (2009). Simvastatin induces apoptosis of fibroblast-like synoviocytes. The open rheumatology journal, 335-40. https://doi.org/10.2174/1874312900903010035
Litinsky, Ira, et al. "Simvastatin induces apoptosis of fibroblast-like synoviocytes." The open rheumatology journal vol. 3 (2009): 35-40. doi: https://doi.org/10.2174/1874312900903010035
Litinsky I, Golan I, Yaron M, Yaron I, Caspi D, Elkayam O. Simvastatin induces apoptosis of fibroblast-like synoviocytes. Open Rheumatol J. 2009 Sep 07;3:35-40. doi: 10.2174/1874312900903010035. PMID: 19771181; PMCID: PMC2746119.
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Open Rheumatol J. 2009 Sep 07;3:35-40. doi: 10.2174/1874312900903010035.

Simvastatin induces apoptosis of fibroblast-like synoviocytes.

The open rheumatology journal

Ira Litinsky, Itshak Golan, Michael Yaron, Ilana Yaron, Dan Caspi, Ori Elkayam

Affiliations

  1. Department of Rheumatology, Sourasky Medical Center and Sackler Faculty of Medicine, University of Tel Aviv, Tel Aviv, Israel.

PMID: 19771181 PMCID: PMC2746119 DOI: 10.2174/1874312900903010035

Abstract

BACKGROUND: Statins (3-Hydroxy-3-methylglutaryl-CoA reductase inhibitors) exert favorable effects on lipoprotein metabolism, but appeared to possess anti-inflammatory properties among others, as suggested by their ability to inhibit collagen-induced arthritis in mice. Their activity in fibroblast-like synovial cells (FLS) has not yet been studied.

OBJECTIVES: To evaluate the effect of varying doses of simvastatin on apoptosis of FLS.

METHODS: Synovial tissue, obtained during total knee replacement due to osteoarthritis, was cut into small pieces and cultured in Petri dishes with test materials, as previously described. FLS were incubated for 48 hours with 1 mumol/ml, 5 mumol/ ml, 15 mumol/ml and 50 mumol/ml of simvastatin. Following incubation, apoptosis was analyzed by two-dimensional flow cytometry (FACS) using annexin V/PI staining according to the manufacturer's instructions.

RESULTS: Different concentrations of simvastatin induced apoptosis of FLS. The level proportion of apoptotic cells of resting or activated with lipopolysaccharide (LPS; 3 mug/ml) FLS, not treated with simvastatin, was 21%. At 48 hours, the rate of apoptosis of activated fibroblasts, incubated with 1 mumol/ml, 5 mumol/ml, 15 and 50 mumol/ml was 22%, 32%, 48% and 41% respectively. Synovial cell viability evaluated by tetrazolium salt XXT was unaffected by the simvastatin concentration used.

CONCLUSION: Varying concentrations of simvastatin induce apoptosis of activated fibroblast-like synoviocytes, suggesting another possible mechanism of anti-inflammatory effects of statins in inflammatory conditions.

Keywords: Apoptosis; fibroblast-like synoviocytes.; inflammation; simvastatin

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