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Tamby MC, Servettaz A, Tamas N, et al. IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts. Biologics. 2008;2(3):583-91doi: 10.2147/btt.s3188.
Tamby, M. C., Servettaz, A., Tamas, N., Reinbolt, J., Caux, F., Meyer, O., Allanore, Y., Kahan, A., Guillevin, L., & Mouthon, L. (2008). IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts. Biologics : targets & therapy, 2(3), 583-91. https://doi.org/10.2147/btt.s3188
Tamby, Mathieu C, et al. "IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts." Biologics : targets & therapy vol. 2,3 (2008): 583-91. doi: https://doi.org/10.2147/btt.s3188
Tamby MC, Servettaz A, Tamas N, Reinbolt J, Caux F, Meyer O, Allanore Y, Kahan A, Guillevin L, Mouthon L. IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts. Biologics. 2008 Sep;2(3):583-91. doi: 10.2147/btt.s3188. PMID: 19707389; PMCID: PMC2721369.
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Biologics. 2008 Sep;2(3):583-91. doi: 10.2147/btt.s3188.

IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts.

Biologics : targets & therapy

Mathieu C Tamby, Amélie Servettaz, Nicolas Tamas, Joseph Reinbolt, Frédéric Caux, Olivier Meyer, Yannick Allanore, André Kahan, Loïc Guillevin, Luc Mouthon

Affiliations

  1. Paris-Descartes University, Faculty of Medicine, UPRES-EA 4058, Paris, France;

PMID: 19707389 PMCID: PMC2721369 DOI: 10.2147/btt.s3188

Abstract

By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age- and gender-matched healthy controls with normal human skin fibroblasts and HEp-2 cells antigens. Serum IgG reactivities of patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies differed significantly from those of healthy controls or systemic sclerosis patients in other groups for reactivity with fibroblast proteins. IgG from patients with antitopoisomerase 1 antibodies bound to a 90 kDa fibroblast band and to a 100 kDa protein band in a HEp-2 cell protein extract. These two bands were further identified as DNA topoisomerase 1. Our results indicate that IgG from patients with diffuse systemic sclerosis bind DNA topoisomerase 1 in normal human fibroblasts extracts.

Keywords: DNA topoisomerase 1; IgG; autoantibodies; fibroblast; systemic sclerosis

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