Display options
Cite
Folta KM, Paul AL, Mayfield JD, et al. 14-3-3 isoforms participate in red light signaling and photoperiodic flowering. Plant Signal Behav. 2008;3(5):304-6doi: 10.4161/psb.3.5.5288.
Folta, K. M., Paul, A. L., Mayfield, J. D., & Ferl, R. J. (2008). 14-3-3 isoforms participate in red light signaling and photoperiodic flowering. Plant signaling & behavior, 3(5), 304-6. https://doi.org/10.4161/psb.3.5.5288
Folta, Kevin M, et al. "14-3-3 isoforms participate in red light signaling and photoperiodic flowering." Plant signaling & behavior vol. 3,5 (2008): 304-6. doi: https://doi.org/10.4161/psb.3.5.5288
Folta KM, Paul AL, Mayfield JD, Ferl RJ. 14-3-3 isoforms participate in red light signaling and photoperiodic flowering. Plant Signal Behav. 2008 May;3(5):304-6. doi: 10.4161/psb.3.5.5288. PMID: 19841653; PMCID: PMC2634265.
Copy Download .nbib Format: NLM
Share it on

Plant Signal Behav. 2008 May;3(5):304-6. doi: 10.4161/psb.3.5.5288.

14-3-3 isoforms participate in red light signaling and photoperiodic flowering.

Plant signaling & behavior

Kevin M Folta, Anna-Lisa Paul, John D Mayfield, Robert J Ferl

Affiliations

  1. Plant Molecular and Cellular Biology Program and Horticultural Sciences Department; University of Florida; Gainesville, Florida USA.

PMID: 19841653 PMCID: PMC2634265 DOI: 10.4161/psb.3.5.5288

Abstract

Members of the 14-3-3 family of proteins participate in signal transduction by modulating flux through various pathways. Potential subfunctionalization within this family has produced a suite of related proteins with diverse client interactions and discrete localization. The associated study assesses the biological roles of two specific 14-3-3 isoforms, using genetic, biochemical and physiological assays to ascertain potential nodes of interaction. Arabidopsis T-DNA insertion mutants representing the nu and mu isoforms exhibited a short, yet clear delay in flowering time on long days. Tests of hypocotyl growth inhibition under narrow bandwidth light indicated a hyposensitivity to red light, while responses to blue and far-red light were normal. These physiological tests suggest a mechanistic link between 14-3-3 proteins, red light sensing, and the pathways that control photoperiodic flowering. The precise entry point into the pathway was assessed using yeast two hybrid assays targeted against specific proteins active in the circadian oscillator, light transduction and photoperiodic flowering. Yeast two hybrid interaction was observed with CONSTANS (CO), and then confirmed with coimmunoprecipitation. Functional interaction with phyB leading to defects in flowering time and direct interaction with CONSTANS circumstantially places these specific 14-3-3 isoforms into the pathway that regulates the transition between vegetative and floral development.

Keywords: isoform specificity; phosphorylation; protein interaction; signaling

References

  1. Bioessays. 2003 Sep;25(9):829-32 - PubMed
  2. Curr Opin Genet Dev. 1996 Aug;6(4):480-7 - PubMed
  3. Plant Mol Biol. 1994 Jul;25(4):659-67 - PubMed
  4. J Biol Chem. 2006 Aug 11;281(32):22875-81 - PubMed
  5. Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:49-73 - PubMed
  6. FEBS Lett. 1996 Aug 12;391(3):252-6 - PubMed
  7. J Biol Chem. 1999 Dec 17;274(51):36774-80 - PubMed
  8. Plant Physiol. 2000 Jan;122(1):235-42 - PubMed
  9. Physiol Plant. 2004 Feb;120(2):173-178 - PubMed
  10. Plant Physiol. 1998 Oct;118(2):551-5 - PubMed
  11. Nature. 2003 Jun 19;423(6942):881-5 - PubMed
  12. FEBS Lett. 1998 Sep 11;435(1):110-4 - PubMed
  13. Science. 2004 Feb 13;303(5660):1003-6 - PubMed
  14. Plant Cell. 1999 Dec;11(12):2379-91 - PubMed
  15. Planta. 2005 Jun;221(3):394-405 - PubMed
  16. FEBS Lett. 1996 Nov 25;398(1):26-30 - PubMed
  17. Mol Biol Cell. 2005 Apr;16(4):1735-43 - PubMed
  18. Curr Opin Plant Biol. 2001 Oct;4(5):436-40 - PubMed
  19. Plant Cell. 2002;14 Suppl:S339-54 - PubMed
  20. Plant Cell. 1999 Aug;11(8):1591-602 - PubMed

Publication Types