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Kabir S, Gerwig GJ. The structural analysis of the O-glycans of the jacalin-bound rabbit immunoglobulin G. Biochem Mol Biol Int. 1997;42(4):769-78doi: 10.1080/15216549700203201.
Kabir, S., & Gerwig, G. J. (1997). The structural analysis of the O-glycans of the jacalin-bound rabbit immunoglobulin G. Biochemistry and molecular biology international, 42(4), 769-78. https://doi.org/10.1080/15216549700203201
Kabir, S, and Gerwig, G J. "The structural analysis of the O-glycans of the jacalin-bound rabbit immunoglobulin G." Biochemistry and molecular biology international vol. 42,4 (1997): 769-78. doi: https://doi.org/10.1080/15216549700203201
Kabir S, Gerwig GJ. The structural analysis of the O-glycans of the jacalin-bound rabbit immunoglobulin G. Biochem Mol Biol Int. 1997 Jul;42(4):769-78. doi: 10.1080/15216549700203201. PMID: 19856294.
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Biochem Mol Biol Int. 1997 Jul;42(4):769-78. doi: 10.1080/15216549700203201.

The structural analysis of the O-glycans of the jacalin-bound rabbit immunoglobulin G.

Biochemistry and molecular biology international

S Kabir, G J Gerwig

Affiliations

  1. Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, Utrecht, The Netherlands.

PMID: 19856294 DOI: 10.1080/15216549700203201

Abstract

Rabbit immunoglobulin G (IgG) is a sialoglycoprotein (2.4% carbohydrate), containing both N-glycolyl (Neu5Gc) and N-acetyl (Neu5Ac) neuraminic acids in a ratio of 87:13. A small fraction of rabbit IgG (about 25% of total IgG) bound to jacalin as demonstrated by affinity chromatography. The jacalin-bound rabbit IgG contained O-linked glycans which were liberated from the protein by beta-elimination and isolated. Two O-glycan structures were determined by 1H-NMR spectroscopy, as being Neu5Gc(alpha2-3)-Gal (beta31-3)-GalNAc-ol and Neu5Ac(alpha2-3)-Gal(beta1-3)-GalNAc-ol in a ratio of 83...n17.

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