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Esumi H, Chin K, Kurashima Y, et al. Reversible cell cycle arrest with concomitant p21/WAF1 overexpression and mitochondrial destruction by nitric oxide. Biochem Mol Biol Int. 1997;42(4):779-87doi: 10.1080/15216549700203211.
Esumi, H., Chin, K., Kurashima, Y., Hasegawa, F., Ogura, T., Yoneda, M., & Ozawa, T. (1997). Reversible cell cycle arrest with concomitant p21/WAF1 overexpression and mitochondrial destruction by nitric oxide. Biochemistry and molecular biology international, 42(4), 779-87. https://doi.org/10.1080/15216549700203211
Esumi, H, et al. "Reversible cell cycle arrest with concomitant p21/WAF1 overexpression and mitochondrial destruction by nitric oxide." Biochemistry and molecular biology international vol. 42,4 (1997): 779-87. doi: https://doi.org/10.1080/15216549700203211
Esumi H, Chin K, Kurashima Y, Hasegawa F, Ogura T, Yoneda M, Ozawa T. Reversible cell cycle arrest with concomitant p21/WAF1 overexpression and mitochondrial destruction by nitric oxide. Biochem Mol Biol Int. 1997 Jul;42(4):779-87. doi: 10.1080/15216549700203211. PMID: 19856295.
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Biochem Mol Biol Int. 1997 Jul;42(4):779-87. doi: 10.1080/15216549700203211.

Reversible cell cycle arrest with concomitant p21/WAF1 overexpression and mitochondrial destruction by nitric oxide.

Biochemistry and molecular biology international

H Esumi, K Chin, Y Kurashima, F Hasegawa, T Ogura, M Yoneda, T Ozawa

Affiliations

  1. Investigative Treatment Division, National Cancer Center Research Institute, Chiba, Japan.

PMID: 19856295 DOI: 10.1080/15216549700203211

Abstract

When human hepatocellular carcinoma Hep-G2 cells were treated with the NO-generating compounds, S-nitroso-N-acetyl-DL-penicillamine(SNAP) or (+/-)-(E)-4-ethyl-2-(E)-hydroxyimino]-5-nitroso-3-hexenamide, cells stopped growing. Most cells were found to be in either G1 or G2/M phase and the dye-exclusion test revealed that the cells were alive. Electron microscopic examination confirmed the integrity of cells and nuclei. Nuclear staining with the DNA-binding dye H33258 revealed that cells did not undergo apoptosis although dramatic changes in mitochondrial morphology were noticed within 6 hr of treatment with SNAP by electron microscopy. Western and northern blot analysesrevealed that cells overexpressed p21/WAF1. The growth arrest was released by withdrawal of the NO-generating compound and cells started to divide within 24 hr after withdrawal of the compounds.

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