Display options
Cite
Joneidi-Jafari H, Daigeler A, Hauser J, et al. A qualitative and quantitative analysis of protein substitution in human burn wounds. Eplasty. 2009;9:e42
Joneidi-Jafari, H., Daigeler, A., Hauser, J., Steinau, H. U., Klatte, W., Fischer, U., & Lehnhardt, M. (2009). A qualitative and quantitative analysis of protein substitution in human burn wounds. Eplasty, 9e42.
Joneidi-Jafari, Hamid, et al. "A qualitative and quantitative analysis of protein substitution in human burn wounds." Eplasty vol. 9 (2009): e42.
Joneidi-Jafari H, Daigeler A, Hauser J, Steinau HU, Klatte W, Fischer U, Lehnhardt M. A qualitative and quantitative analysis of protein substitution in human burn wounds. Eplasty. 2009 Sep 29;9:e42. PMID: 19865469; PMCID: PMC2754910.
Copy Download .nbib Format: NLM
Share it on

Eplasty. 2009 Sep 29;9:e42.

A qualitative and quantitative analysis of protein substitution in human burn wounds.

Eplasty

Hamid Joneidi-Jafari, Adrien Daigeler, Joerg Hauser, Hans-Ulrich Steinau, Walter Klatte, Ulrich Fischer, Marcus Lehnhardt

Affiliations

  1. Department of Surgery, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Germany. [email protected]

PMID: 19865469 PMCID: PMC2754910

Abstract

OBJECTIVE: In major burn wounds of more than 15% total burn surface area mediator-associated reactions lead to capillary leak resulting in critical condition. Little is known about the efficiency of protein substitution. We quantified and qualified the systemic and local protein loss in burn patients during protein substitution, comparing fresh frozen plasma and the human serum protein solution Biseko.

METHODS: In 40 patients suffering from second-degree burn wounds with the total burn surface area between 20% and 60%, immediately after admission a defined wound surface area was enclosed with in a wound chamber. Wound fluid and serum samples were collected in 8 hour intervals for 2 days. Samples were analyzed for total protein, albumin, immunoglobulins -A, -G, -M, clotting parameters, c-reactive protein, and white blood cells. Protein substitution started 24 hour posttrauma. In a randomized pattern, patients received equal volumes of fresh frozen plasma or Biseko.

RESULTS: Total protein and albumin accumulated in high concentrations in wound fluid. With beginning of fresh frozen plasma substitution on day 2 posttrauma, serum total protein (1.7 g-3.9 g) and albumin (1.3 g-3.4 g) concentrations increased. Substitution of Biseko resulted in a stronger increase (serum total protein 1.8 g to 4.5 g, albumin 0.9 g to 3.4 g). Wound fluid concentrations revealed similar change patterns. Immunoglobulins showed higher serum levels in the Biseko group. C-reactive protein and white blood cell values indicated a lower immunological reaction in the Biseko group.

CONCLUSIONS: Substitution of human protein solutions such as Biseko can result in significantly higher serum protein and albumin concentrations as well as lower infection parameters. Higher serum immunoglobulins could help to decrease potential immunodeficiency.

References

  1. World J Surg. 1992 Jan-Feb;16(1):53-6 - PubMed
  2. Acta Physiol Scand Suppl. 1979;463:111-22 - PubMed
  3. Crit Care Med. 1981 Jul;9(7):503-8 - PubMed
  4. Surgery. 1984 Feb;95(2):136-44 - PubMed
  5. Crit Care Med. 1983 Nov;11(11):839-50 - PubMed
  6. J Clin Anesth. 1992 Jan-Feb;4(1):4-10 - PubMed
  7. Burns. 2005 Mar;31(2):159-67 - PubMed
  8. J Trauma. 1979 Nov;19(11 Suppl):864-5 - PubMed
  9. Surg Gynecol Obstet. 1972 Aug;135(2):257-60 - PubMed
  10. J Trauma. 1986 Jan;26(1):34-9 - PubMed
  11. J Surg Res. 1980 Feb;28(2):110-7 - PubMed
  12. Intensive Care Med. 2004 Dec;30(12):2222-9 - PubMed
  13. Arch Surg. 1997 Feb;132(2):158-61 - PubMed
  14. Resuscitation. 1991 Apr;21(2-3):137-80 - PubMed
  15. Jpn J Clin Oncol. 2003 Jun;33(6):309-13 - PubMed
  16. Crit Care Med. 1999 Jan;27(1):34-5 - PubMed
  17. Am J Physiol. 1982 Nov;243(5):H803-9 - PubMed
  18. J Burn Care Rehabil. 1996 Sep-Oct;17(5):379-83 - PubMed
  19. Z Gastroenterol. 2001 Jun;39 Suppl 2:24-7 - PubMed
  20. World J Surg. 1992 Jan-Feb;16(1):30-6 - PubMed
  21. J Wound Care. 2005 Jun;14(6):256-8 - PubMed
  22. Am J Nephrol. 2003 Jan-Feb;23(1):55-60 - PubMed
  23. Eur J Anaesthesiol. 2003 Jun;20(6):429-42 - PubMed
  24. Circ Res. 1981 Nov;49(5):1173-80 - PubMed
  25. Burns. 1991 Dec;17(6):484-9 - PubMed
  26. J Clin Apher. 1987;3(3):185-90 - PubMed
  27. Arch Surg. 1972 Sep;105(3):477-82 - PubMed
  28. Transfus Apher Sci. 2004 Aug;31(1):67-73 - PubMed
  29. Shock. 1996 Jan;5(1):4-16 - PubMed
  30. Crit Care Med. 2003 Aug;31(8 Suppl):S502-11 - PubMed
  31. Crit Care Med. 2002 Nov;30(11 Suppl):S500-14 - PubMed
  32. J Indian Med Assoc. 1996 Apr;94(4):133-4 - PubMed
  33. Scand J Plast Reconstr Surg. 1979;13(1):29-38 - PubMed
  34. Transfus Apher Sci. 2006 Aug;35(1):83-90 - PubMed

Publication Types