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Gorovsky MA, Woodard J. STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS: I. RNA Synthesis in Macro- and Micronuclei. J Cell Biol. 1969;42(3):673-82doi: 10.1083/jcb.42.3.673.
Gorovsky, M. A., & Woodard, J. (1969). STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS: I. RNA Synthesis in Macro- and Micronuclei. The Journal of cell biology, 42(3), 673-82. https://doi.org/10.1083/jcb.42.3.673
Gorovsky, M A, and Woodard, J. "STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS: I. RNA Synthesis in Macro- and Micronuclei." The Journal of cell biology vol. 42,3 (1969): 673-82. doi: https://doi.org/10.1083/jcb.42.3.673
Gorovsky MA, Woodard J. STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS: I. RNA Synthesis in Macro- and Micronuclei. J Cell Biol. 1969 Sep 01;42(3):673-82. doi: 10.1083/jcb.42.3.673. PMID: 19866732; PMCID: PMC2107700.
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J Cell Biol. 1969 Sep 01;42(3):673-82. doi: 10.1083/jcb.42.3.673.

STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS: I. RNA Synthesis in Macro- and Micronuclei.

The Journal of cell biology

M A Gorovsky, J Woodard

Affiliations

  1. Whitman Laboratory, the Department of Biology, The University of Chicago, Chicago, Illinois 60637.

PMID: 19866732 PMCID: PMC2107700 DOI: 10.1083/jcb.42.3.673

Abstract

Tetrahymena in the log phase of growth were pulse labeled with uridine-(3)H, fixed in acetic-alcohol, extracted with DNase, and embedded in Epon. 0.5-micro sections were cut, coated with Kodak NTB-2 emulsion, and developed after suitable exposures. Grains were counted above macronuclei, above 1000 micronuclei, and above 1000 micronucleus-sized "blanks" which were situated next to micronuclei in the visual field by means of a camera lucida. An analysis of grain counts showed that micronuclei were less than (1/2)(000) as active as macronuclei on the basis of grains per nucleus. Since micronuclei contained, on the average, about (1/2)(0) as much DNA as macronuclei, micronuclear DNA had less than 1% of the specific activity of macronuclear DNA in RNA synthesis. However, even this small amount of apparent incorporation was not significantly different from zero. Comparisons of the frequency distributions of labeled micronuclei with those of micronuclear "blanks" showed no evidence of a small population of labeled nuclei such as might be expected if micronuclei synthesized RNA for only a brief portion of the cell cycle. We conclude from these studies that there is no detectable RNA synthesis in Tetrahymena micronuclei during vegetative growth and reproduction.

References

  1. J Biophys Biochem Cytol. 1961 Feb;9:409-14 - PubMed
  2. J R Microsc Soc. 1964 Jun;83:161-7 - PubMed
  3. Lab Invest. 1952;1(2):250-65 - PubMed
  4. J Cell Biol. 1967 May;33(2):281-5 - PubMed
  5. J Protozool. 1964 Aug;11:370-8 - PubMed
  6. J Biophys Biochem Cytol. 1955 Jan;1(1):17-28 - PubMed
  7. Chromosoma. 1952;5(4):341-62 - PubMed
  8. J Cell Biol. 1965 Dec;27(3):519-29 - PubMed
  9. J Exp Zool. 1967 Aug;165(3):395-417 - PubMed

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