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Tiselius A, Kabat EA. AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS. J Exp Med. 1939;69(1):119-31doi: 10.1084/jem.69.1.119.
Tiselius, A., & Kabat, E. A. (1939). AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS. The Journal of experimental medicine, 69(1), 119-31. https://doi.org/10.1084/jem.69.1.119
Tiselius, A, and Kabat, E A. "AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS." The Journal of experimental medicine vol. 69,1 (1939): 119-31. doi: https://doi.org/10.1084/jem.69.1.119
Tiselius A, Kabat EA. AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS. J Exp Med. 1939 Jan 01;69(1):119-31. doi: 10.1084/jem.69.1.119. PMID: 19870831; PMCID: PMC2133726.
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J Exp Med. 1939 Jan 01;69(1):119-31. doi: 10.1084/jem.69.1.119.

AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS.

The Journal of experimental medicine

A Tiselius, E A Kabat

Affiliations

  1. Institute of Physical Chemistry, University of Upsala, Upsala, Sweden.

PMID: 19870831 PMCID: PMC2133726 DOI: 10.1084/jem.69.1.119

Abstract

1. Antibody produced in the horse migrates as a new serum component between the beta and gamma components, whereas rabbit antibody is electrophoretically identical with the gamma globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the gamma globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbed and absorbed sera. Antibody solutions of high purity can be obtained by electrophoretic isolation of the gamma globulin of rabbit antisera in which the percentage of antibody to total gamma globulin is high. 3. The isoelectric points of pig, cow, horse, and rabbit antibodies have been determined. 4. In horse sera prolonged immunization is accompanied by the formation of another antibody component of lower mobility.

References

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