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Kurpinski K, Chu J, Wang D, et al. Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-beta1. Cell Mol Bioeng. 2009;2(4):606-614doi: 10.1007/s12195-009-0090-6.
Kurpinski, K., Chu, J., Wang, D., & Li, S. (2009). Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-beta1. Cellular and molecular bioengineering, 2(4), 606-614. https://doi.org/10.1007/s12195-009-0090-6
Kurpinski, Kyle, et al. "Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-beta1." Cellular and molecular bioengineering vol. 2,4 (2009): 606-614. doi: https://doi.org/10.1007/s12195-009-0090-6
Kurpinski K, Chu J, Wang D, Li S. Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-beta1. Cell Mol Bioeng. 2009 Dec;2(4):606-614. doi: 10.1007/s12195-009-0090-6. Epub 2009 Oct 24. PMID: 20037637; PMCID: PMC2792360.
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Cell Mol Bioeng. 2009 Dec;2(4):606-614. doi: 10.1007/s12195-009-0090-6. Epub 2009 Oct 24.

Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-beta1.

Cellular and molecular bioengineering

Kyle Kurpinski, Julia Chu, Daojing Wang, Song Li

PMID: 20037637 PMCID: PMC2792360 DOI: 10.1007/s12195-009-0090-6

Abstract

Mesenchymal stem cells (MSCs) are a potential source of smooth muscle cells (SMCs) for constructing tissue-engineered vascular grafts. However, the details of how specific combinations of vascular microenvironmental factors regulate MSCs are not well understood. Previous studies have suggested that both mechanical stimulation with uniaxial cyclic strain and chemical stimulation with transforming growth factor-beta1 (TGF-beta1) can induce smooth muscle markers in MSCs. In this study, we investigated the combined effects of uniaxial cyclic strain and TGF-beta1 stimulation on MSCs. By using a proteomic analysis, we found differential regulation of several proteins and genes, such as the up-regulation of TGF-beta1-induced protein ig-h3 (BGH3) protein levels by TGF-beta1 and up-regulation of calponin 3 protein level by cyclic strain. At the gene expression level, BGH3 was induced by TGF-beta1, but calponin 3 was not significantly regulated by mechanical strain or TGF-beta1, which was in contrast to the synergistic up-regulation of calponin 1 gene expression by cyclic strain and TGF-beta1. Further experiments with cycloheximide treatment suggested that the up-regulation of calponin 3 by cyclic strain was at post-transcriptional level. The results in this study suggest that both mechanical stimulation and TGF-beta1 signaling play unique and important roles in the regulation of MSCs at both transcriptional and post-transcriptional levels, and that a precise combination of microenvironmental cues may promote MSC differentiation.

References

  1. Hypertension. 2000 Sep;36(3):319-24 - PubMed
  2. Nature. 2002 Jul 4;418(6893):41-9 - PubMed
  3. FASEB J. 2008 Jun;22(6):1635-48 - PubMed
  4. Trends Cardiovasc Med. 2000 Apr;10(3):132-7 - PubMed
  5. Annu Rev Biomed Eng. 2001;3:225-43 - PubMed
  6. Tissue Eng. 2004 Mar-Apr;10(3-4):361-9 - PubMed
  7. Trends Mol Med. 2001 Jun;7(6):259-64 - PubMed
  8. Tissue Eng. 1998 Winter;4(4):415-28 - PubMed
  9. J Vasc Res. 1998 Mar-Apr;35(2):93-103 - PubMed
  10. Biochem Biophys Res Commun. 1995 Dec 5;217(1):238-44 - PubMed
  11. Exp Cell Res. 1998 Jan 10;238(1):265-72 - PubMed
  12. Nature. 2003 Oct 9;425(6958):577-84 - PubMed
  13. Biotechnol Bioeng. 2004 Nov 5;88(3):359-68 - PubMed
  14. EMBO J. 2000 Apr 17;19(8):1745-54 - PubMed
  15. Exp Cell Res. 2002 Aug 1;278(1):72-83 - PubMed
  16. Bone. 2005 Feb;36(2):232-42 - PubMed
  17. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11915-20 - PubMed
  18. J Biol Chem. 2004 Oct 15;279(42):43725-34 - PubMed
  19. Arch Surg. 1988 Oct;123(10):1233-6 - PubMed
  20. Science. 1999 Apr 2;284(5411):143-7 - PubMed
  21. J Cell Sci. 2001 Dec;114(Pt 24):4359-69 - PubMed
  22. Cell Mol Life Sci. 2008 Jul;65(14):2244-55 - PubMed
  23. DNA Cell Biol. 1994 Jun;13(6):571-84 - PubMed
  24. Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16095-100 - PubMed
  25. J Cell Physiol. 1993 Dec;157(3):615-24 - PubMed

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