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Chihara S, Hayden MK, Minogue-Corbett E, et al. Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar. Int J Microbiol. 2009;2009:636502doi: 10.1155/2009/636502.
Chihara, S., Hayden, M. K., Minogue-Corbett, E., & Singh, K. (2009). Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar. International journal of microbiology, 2009636502. https://doi.org/10.1155/2009/636502
Chihara, Shingo, et al. "Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar." International journal of microbiology vol. 2009 (2009): 636502. doi: https://doi.org/10.1155/2009/636502
Chihara S, Hayden MK, Minogue-Corbett E, Singh K. Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar. Int J Microbiol. 2009;2009:636502. doi: 10.1155/2009/636502. Epub 2009 Jan 11. PMID: 20016679; PMCID: PMC2777004.
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Int J Microbiol. 2009;2009:636502. doi: 10.1155/2009/636502. Epub 2009 Jan 11.

Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar.

International journal of microbiology

Shingo Chihara, Mary K Hayden, Eileen Minogue-Corbett, Kamaljit Singh

Affiliations

  1. Department of Medicine, Section of Infectious Diseases, Rush University Medical Center, 1653 W. Congress Parkway, Chicago, IL 60612, USA.

PMID: 20016679 PMCID: PMC2777004 DOI: 10.1155/2009/636502

Abstract

The ability to rapidly differentiate coagulase-negative staphylococcus (CoNS) from Staphylococcus aureus and to determine methicillin resistance is important as it affects the decision to treat empiric antibiotic selection. The objective of this study was to evaluate CHROMagar S. aureus and CHROMagar MRSA (Becton Dickinson) for rapid identification of Staphylococcus spp. directly from blood cultures. Consecutive blood culture bottles (BacT Alert 3D SA and SN, bioMérieux) growing gram-positive cocci in clusters were evaluated. An aliquot was plated onto CHROMagar MRSA (C-MRSA) and CHROMagar S. aureus (C-SA) plates, which were read at 12 to 16 hours. C-SA correctly identified 147/147 S. aureus (100% sensitivity); 2 CoNS were misidentified as S. aureus (98% specificity). C-MRSA correctly identified 74/77 MRSA (96% sensitivity). None of the MSSA isolates grew on C-MRSA (100% specificity). In conclusion, CHROMagar is a rapid and sensitive method to distinguish MRSA, MSSA, and coagulase-negative Staphylococcus and may decrease time of reporting positive results.

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