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Earhart CM, Wilson RJ, White RL, et al. Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation. J Magn Magn Mater. 2009;321(10):1436-1439doi: 10.1016/j.jmmm.2009.02.062.
Earhart, C. M., Wilson, R. J., White, R. L., Pourmand, N., & Wang, S. X. (2009). Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation. Journal of magnetism and magnetic materials, 321(10), 1436-1439. https://doi.org/10.1016/j.jmmm.2009.02.062
Earhart, Christopher M, et al. "Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation." Journal of magnetism and magnetic materials vol. 321,10 (2009): 1436-1439. doi: https://doi.org/10.1016/j.jmmm.2009.02.062
Earhart CM, Wilson RJ, White RL, Pourmand N, Wang SX. Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation. J Magn Magn Mater. 2009 May;321(10):1436-1439. doi: 10.1016/j.jmmm.2009.02.062. PMID: 20161248; PMCID: PMC2707938.
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J Magn Magn Mater. 2009 May;321(10):1436-1439. doi: 10.1016/j.jmmm.2009.02.062.

Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation.

Journal of magnetism and magnetic materials

Christopher M Earhart, Robert J Wilson, Robert L White, Nader Pourmand, Shan X Wang

Affiliations

  1. Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305.

PMID: 20161248 PMCID: PMC2707938 DOI: 10.1016/j.jmmm.2009.02.062

Abstract

A microfabricated magnetic sifter has been designed and fabricated for applications in biological sample preparation. The device enables high-throughput, high-gradient magnetic separation of magnetic nanoparticles by utilizing columnar fluid flow through a dense array (~5000/mm(2)) of micropatterned slots in a magnetically soft membrane. The potential of the sifter for separation of magnetic nanoparticles conjugated with capture antibodies is demonstrated through quantitative separation experiments with CD138-labelled MACS nanoparticles. Capture efficiencies ranging from 28-37% and elution efficiencies greater than 73% were measured for a single pass through the sifter.

References

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