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Gamage P, Basel MT, Lovell K, et al. Poly-N-Isopropylacrylamide/acrylic Acid Copolymers for the Generation of Nanostructures at Mica Surfaces and as Hydrophobic Host Systems for the Porin MspA from Mycobacterium smegmatis. J Phys Chem C Nanomater Interfaces. 2009;113(37):16485-16494doi: 10.1021/jp9057687.
Gamage, P., Basel, M. T., Lovell, K., Pokhrel, M. R., Battle, D., Ito, T., Pavlenok, M., Niederweis, M., & Bossmann, S. H. (2009). Poly-N-Isopropylacrylamide/acrylic Acid Copolymers for the Generation of Nanostructures at Mica Surfaces and as Hydrophobic Host Systems for the Porin MspA from Mycobacterium smegmatis. The journal of physical chemistry. C, Nanomaterials and interfaces, 113(37), 16485-16494. https://doi.org/10.1021/jp9057687
Gamage, Pubudu, et al. "Poly-N-Isopropylacrylamide/acrylic Acid Copolymers for the Generation of Nanostructures at Mica Surfaces and as Hydrophobic Host Systems for the Porin MspA from Mycobacterium smegmatis." The journal of physical chemistry. C, Nanomaterials and interfaces vol. 113,37 (2009): 16485-16494. doi: https://doi.org/10.1021/jp9057687
Gamage P, Basel MT, Lovell K, Pokhrel MR, Battle D, Ito T, Pavlenok M, Niederweis M, Bossmann SH. Poly-N-Isopropylacrylamide/acrylic Acid Copolymers for the Generation of Nanostructures at Mica Surfaces and as Hydrophobic Host Systems for the Porin MspA from Mycobacterium smegmatis. J Phys Chem C Nanomater Interfaces. 2009 Sep 17;113(37):16485-16494. doi: 10.1021/jp9057687. PMID: 20161351; PMCID: PMC2776743.
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J Phys Chem C Nanomater Interfaces. 2009 Sep 17;113(37):16485-16494. doi: 10.1021/jp9057687.

Poly-N-Isopropylacrylamide/acrylic Acid Copolymers for the Generation of Nanostructures at Mica Surfaces and as Hydrophobic Host Systems for the Porin MspA from Mycobacterium smegmatis.

The journal of physical chemistry. C, Nanomaterials and interfaces

Pubudu Gamage, Matthew T Basel, Kimberly Lovell, Megh Raj Pokhrel, Deletria Battle, Takashi Ito, Mikhail Pavlenok, Michael Niederweis, Stefan H Bossmann

Affiliations

  1. Kansas State University, Department of Chemistry and Terry C. Johnson Center for Basic Cancer Research, The Midwest Institute for Comparative Stem Cell Biology, 213 CBC Building, Manhattan, KS 66506-0401, USA, [email protected].

PMID: 20161351 PMCID: PMC2776743 DOI: 10.1021/jp9057687

Abstract

The work presented here aims at utilizing poly-N-isopropyl-acrylamide/acrylic acid copolymers to create nanostructured layers on mica surfaces by a simple spin-casting procedure. The average composition of the copolymers determined by elemental analysis correlates excellently with the feed composition indicating that the radical polymerization process is statistical. The resulting surfaces were characterized by Atomic Force Microscopy (magnetic AC-mode) at the copolymer/air interface. Postpolymerization modification of the acrylic acid functions with perfluoro-octyl-iodide decreased the tendency towards spontaneous formation of nanopores. Crosslinking of individual polymer chains permitted the generation of ultraflat layers, which hosted the mycobacterial channel protein MspA, without compromising its channel function. The comparison of copolymers of very similar chemical composition that have been prepared by living radical polymerization and classic radical polymerization indicated that differences in polydispersity played only a minor role when poly-N-isopropyl-acrylamide/acrylic acid copolymers were spincast, but a major role when copolymers featuring the strongly hydrophobic perfluoro-octyl-labels were used. The mean pore diameters were 23.8+/-4.4 nm for P[(NIPAM)(95.5)-co-(AA)(4.5)] (PDI (polydispersity index)=1.55) and 21.8+/-4.2 nm for P[(NIPAM)(95.3)-co-(AA)(4.7)] (PDI=1.25). The depth of the nanopores was approx. 4 nm. When depositing P[(NIPAM)(95)-co-(AA)(2.8)-AAC(8)F(17 2.2)] (PDI=1.29) on Mica, the resulting mean pore diameter was 35.8+/-7.1 nm, with a depth of only 2 nm.

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