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Mitsuhashi M, Peel D, Ziogas A, et al. Enhanced Expression of Radiation-induced Leukocyte CDKN1A mRNA in Multiple Primary Breast Cancer Patients: Potential New Marker of Cancer Susceptibility. Biomark Insights. 2009;4:201-9doi: 10.4137/bmi.s3774.
Mitsuhashi, M., Peel, D., Ziogas, A., & Anton-Culver, H. (2009). Enhanced Expression of Radiation-induced Leukocyte CDKN1A mRNA in Multiple Primary Breast Cancer Patients: Potential New Marker of Cancer Susceptibility. Biomarker insights, 4201-9. https://doi.org/10.4137/bmi.s3774
Mitsuhashi, Masato, et al. "Enhanced Expression of Radiation-induced Leukocyte CDKN1A mRNA in Multiple Primary Breast Cancer Patients: Potential New Marker of Cancer Susceptibility." Biomarker insights vol. 4 (2009): 201-9. doi: https://doi.org/10.4137/bmi.s3774
Mitsuhashi M, Peel D, Ziogas A, Anton-Culver H. Enhanced Expression of Radiation-induced Leukocyte CDKN1A mRNA in Multiple Primary Breast Cancer Patients: Potential New Marker of Cancer Susceptibility. Biomark Insights. 2009 Dec 22;4:201-9. doi: 10.4137/bmi.s3774. PMID: 20072670; PMCID: PMC2805425.
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Biomark Insights. 2009 Dec 22;4:201-9. doi: 10.4137/bmi.s3774.

Enhanced Expression of Radiation-induced Leukocyte CDKN1A mRNA in Multiple Primary Breast Cancer Patients: Potential New Marker of Cancer Susceptibility.

Biomarker insights

Masato Mitsuhashi, David Peel, Argyrios Ziogas, Hoda Anton-Culver

Affiliations

  1. Hitachi Chemical Research Center, Inc., Irvine, CA, USA.

PMID: 20072670 PMCID: PMC2805425 DOI: 10.4137/bmi.s3774

Abstract

This study was designed to discover blood biomarkers of cancer susceptibility using invasive multiple (n = 21), single primary breast cancer (n = 21), and control subjects (n = 20). Heparinized whole blood was incubated at 37 degrees C for 2 hours after 0-10 Gy of radiation, then cell cycle arrest marker CDKN1A and apoptosis marker BBC3 mRNA were quantified. This epidemiological study was practically feasible because radiation-induced mRNA was preserved for at least 1 day whenever blood was stored at 4 degrees C (r(2) = 0.901). Moreover, blood could be stored frozen after radiation treatment (r(2) = 0.797). Radiation-induced CDKN1A and BBC3 mRNA were dose dependent, and the degree of induction of CDKN1A was correlated with that of BBC3 (r(2) = 0.679). Interestingly, multiple primary cases showed higher induction of CDKN1A mRNA than single primary and control groups, whereas BBC3 did not show such differences. The results suggested that cancer susceptibility represented by the multiple primary breast cancer cases was related to over-reaction of CDKN1A mRNA, not BBC3. The study also suggests that ex vivo gene expression analysis could potentially be used as a new tool in epidemiological studies for cancer and radiation sensitivity research.

Keywords: BBC3; CDKN1A; DNA damage; cancer susceptibility Mitsuhashi et al; multiple primary cancer

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