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El-Bondkly AM, Abd-Alla HI, Shaaban M, et al. The Electrospray Ionization - Mass Spectra of Erythromycin A Obtained from a Marine Streptomyces sp. Mutant. Indian J Pharm Sci. 2008;70(3):310-9doi: 10.4103/0250-474X.42979.
El-Bondkly, A. M., Abd-Alla, H. I., Shaaban, M., & Shaaban, K. A. (2008). The Electrospray Ionization - Mass Spectra of Erythromycin A Obtained from a Marine Streptomyces sp. Mutant. Indian journal of pharmaceutical sciences, 70(3), 310-9. https://doi.org/10.4103/0250-474X.42979
El-Bondkly, A M, et al. "The Electrospray Ionization - Mass Spectra of Erythromycin A Obtained from a Marine Streptomyces sp. Mutant." Indian journal of pharmaceutical sciences vol. 70,3 (2008): 310-9. doi: https://doi.org/10.4103/0250-474X.42979
El-Bondkly AM, Abd-Alla HI, Shaaban M, Shaaban KA. The Electrospray Ionization - Mass Spectra of Erythromycin A Obtained from a Marine Streptomyces sp. Mutant. Indian J Pharm Sci. 2008 May-Jun;70(3):310-9. doi: 10.4103/0250-474X.42979. PMID: 20046738; PMCID: PMC2792501.
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Indian J Pharm Sci. 2008 May-Jun;70(3):310-9. doi: 10.4103/0250-474X.42979.

The Electrospray Ionization - Mass Spectra of Erythromycin A Obtained from a Marine Streptomyces sp. Mutant.

Indian journal of pharmaceutical sciences

A M El-Bondkly, Howaida I Abd-Alla, M Shaaban, K A Shaaban

PMID: 20046738 PMCID: PMC2792501 DOI: 10.4103/0250-474X.42979

Abstract

In our ongoing search for production improvements of bioactive secondary metabolites from marine Streptomyces through the induction of mutations using UV light, out of 145 isolates, mutant 10/14 was able to produce potent antibacterial metabolites other than the parent strain as established by chromatographic analysis. Up-scaling fermentation of mutant 10/14, followed by working up and isolation delivered five metabolites, phenazine, 1-acetyl-beta -carboline, perlolyrin and erythromycin A, along with an oily substance. The latter two compounds were responsible for the antibacterial activity of the strain. In this article, we discuss with the mutation of the marine Streptomyces sp. AH2, bioactivity evaluation, fermentation and isolation of the microbial metabolites. Moreover, we study to first time in detail the 1D and 2D NMR and ESI MS data including ESI MS(2) and MS(3) patterns combined with HRESI MS of erythromycin A.

Keywords: ESI-MS/MS; Marine Streptomyces mutation; bioactivity evaluation; erythromycin A

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