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Farnebo S, Lars-Erik K, Ingrid S, et al. Continuous assessment of concentrations of cytokines in experimental injuries of the extremity. Int J Clin Exp Med. 2009;2(4):354-62
Farnebo, S., Lars-Erik, K., Ingrid, S., Sjögren, F., & Folke, S. (2009). Continuous assessment of concentrations of cytokines in experimental injuries of the extremity. International journal of clinical and experimental medicine, 2(4), 354-62.
Farnebo, Simon, et al. "Continuous assessment of concentrations of cytokines in experimental injuries of the extremity." International journal of clinical and experimental medicine vol. 2,4 (2009): 354-62.
Farnebo S, Lars-Erik K, Ingrid S, Sjögren F, Folke S. Continuous assessment of concentrations of cytokines in experimental injuries of the extremity. Int J Clin Exp Med. 2009 Nov 26;2(4):354-62. PMID: 20057979; PMCID: PMC2802052.
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Int J Clin Exp Med. 2009 Nov 26;2(4):354-62.

Continuous assessment of concentrations of cytokines in experimental injuries of the extremity.

International journal of clinical and experimental medicine

Simon Farnebo, Karlander Lars-Erik, Steinwall Ingrid, Florence Sjögren, Sjöberg Folke

PMID: 20057979 PMCID: PMC2802052

Abstract

Background. Inflammation plays an important part in the healing process. Little is known about the extent local inflammatory trauma response interacts with the central circulation and inflammation produced by central organs. The aim of the present study was to examine whether high cut-off microdialysis catheters offer potential to in real time assess interstitial cytokines variations in conjunction to markers of metabolism distal to a blunt vascular contusion. Methods. In a standardised contusion trauma model, microdialysis catheters (high MW (100kDa)) were inserted in the gracilis muscle distal to the trauma for the local assessment of IL-6, IL-8, TNF-alpha, total protein and the metabolic mediators (glycerol, puruvate and lactate). The contra lateral uninjured leg served as control of the centrally mediated inflammation propagated to the extremities. Results. The trauma led to a significant and quantitatively large (8-10 fold) increase in inflammatory cytokines (IL6 and 8) as measured both in the injured and control legs. There was only a minor, and not significant increase in concentrations of cytokines in the injured leg compared to the control leg.. There were no signs of ischemia in either leg. Conclusion. The new finding in this study is that both central, and local, inflammatory responses as well as metabolic mediators may be assessed continuously in skeletal muscle tissue distal to a major injury in an animal model. The findings suggest that the large trauma elicits a generalised inflammatory response to trauma rather than propagating a local one distal to the trauma.

Keywords: Blunt trauma; inflammation; microcirculation; microdialysis; rat

References

  1. J Trauma. 1997 Mar;42(3):446-54; discussion 454-5 - PubMed
  2. J Trauma. 1999 Aug;47(2):358-64 - PubMed
  3. Ann Surg. 1996 Nov;224(5):647-64 - PubMed
  4. J Physiol. 2006 May 15;573(Pt 1):225-36 - PubMed
  5. Neurosurgery. 2005 Jun;56(6):1264-8; discussion 1268-70 - PubMed
  6. Lymphokine Res. 1990 Summer;9(2):231-8 - PubMed
  7. J Appl Physiol (1985). 2005 Feb;98(2):477-81 - PubMed
  8. Br J Dermatol. 2002 Mar;146(3):375-82 - PubMed
  9. J Trauma. 2000 Feb;48(2):286-91 - PubMed
  10. J Neuroimmunol. 1991 Sep;33(3):227-36 - PubMed
  11. Shock. 2003 Apr;19(4):299-304 - PubMed
  12. Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H735-44 - PubMed
  13. Scand J Plast Reconstr Surg Hand Surg. 1991;25(1):1-4 - PubMed
  14. Muscle Nerve. 1998 Jun;21(6):819-22 - PubMed
  15. Scand J Plast Reconstr Surg Hand Surg. 1994 Mar;28(1):19-23 - PubMed
  16. Anal Chem. 2007 Mar 1;79(5):1816-24 - PubMed
  17. Acta Derm Venereol. 2009;89(5):459-65 - PubMed
  18. Shock. 2005 Jun;23(6):488-93 - PubMed
  19. J Trauma. 1991 Aug;31(8):1063-7 - PubMed
  20. J Trauma. 1994 Jul;37(1):91-8; discussion 98-9 - PubMed
  21. Cardiovasc Res. 1995 Nov;30(5):711-5 - PubMed
  22. Ann Surg. 1993 Dec;218(6):769-76 - PubMed
  23. Microvasc Res. 2005 Jul;70(1-2):111-5 - PubMed
  24. Am J Physiol. 1991 Jan;260(1 Pt 2):R167-71 - PubMed

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