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Ranjan RK, Rajagopal K. Efficient ligation and cloning of DNA fragments with 2-bp overhangs. Anal Biochem. 2010;402(1):91-2doi: 10.1016/j.ab.2010.03.020.
Ranjan, R. K., & Rajagopal, K. (2010). Efficient ligation and cloning of DNA fragments with 2-bp overhangs. Analytical biochemistry, 402(1), 91-2. https://doi.org/10.1016/j.ab.2010.03.020
Ranjan, Rajiv Kumar, and Rajagopal, Kammara. "Efficient ligation and cloning of DNA fragments with 2-bp overhangs." Analytical biochemistry vol. 402,1 (2010): 91-2. doi: https://doi.org/10.1016/j.ab.2010.03.020
Ranjan RK, Rajagopal K. Efficient ligation and cloning of DNA fragments with 2-bp overhangs. Anal Biochem. 2010 Jul 01;402(1):91-2. doi: 10.1016/j.ab.2010.03.020. Epub 2010 Mar 15. PMID: 20298672.
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Anal Biochem. 2010 Jul 01;402(1):91-2. doi: 10.1016/j.ab.2010.03.020. Epub 2010 Mar 15.

Efficient ligation and cloning of DNA fragments with 2-bp overhangs.

Analytical biochemistry

Rajiv Kumar Ranjan, Kammara Rajagopal

Affiliations

  1. Institute of Microbial Technology, Sector-39a, Chandigarh 160036, India.

PMID: 20298672 DOI: 10.1016/j.ab.2010.03.020

Abstract

Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers.

2010 Elsevier Inc. All rights reserved.

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