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Yee CH, Aoki S, Uchihashi K, et al. The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase. Acta Histochem Cytochem. 2010;43(1):1-7doi: 10.1267/ahc.09036.
Yee, C. H., Aoki, S., Uchihashi, K., Matsunobu, A., Yamasaki, F., Misago, N., Piao, M., Tetsuji, U., Yonemitsu, N., Sugihara, H., & Toda, S. (2010). The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase. Acta histochemica et cytochemica, 43(1), 1-7. https://doi.org/10.1267/ahc.09036
Yee, Chong Hong, et al. "The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase." Acta histochemica et cytochemica vol. 43,1 (2010): 1-7. doi: https://doi.org/10.1267/ahc.09036
Yee CH, Aoki S, Uchihashi K, Matsunobu A, Yamasaki F, Misago N, Piao M, Tetsuji U, Yonemitsu N, Sugihara H, Toda S. The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase. Acta Histochem Cytochem. 2010 Feb 27;43(1):1-7. doi: 10.1267/ahc.09036. Epub 2010 Feb 04. PMID: 20300218; PMCID: PMC2840220.
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Acta Histochem Cytochem. 2010 Feb 27;43(1):1-7. doi: 10.1267/ahc.09036. Epub 2010 Feb 04.

The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase.

Acta histochemica et cytochemica

Chong Hong Yee, Shigehisa Aoki, Kazuyoshi Uchihashi, Aki Matsunobu, Fumio Yamasaki, Noriyuki Misago, Meihua Piao, Uemura Tetsuji, Nobuhisa Yonemitsu, Hajime Sugihara, Shuji Toda

Affiliations

  1. Department of Pathology & Microbiology, Faculty of Medicine, Saga University, Japan.

PMID: 20300218 PMCID: PMC2840220 DOI: 10.1267/ahc.09036

Abstract

The air-liquid interface (ALI) is a common microenvironment of the skin, but it is unknown whether the ALI affects melanoma cell behaviors. Using a collagen gel invasion assay, immunohistochemistry, and Western blots, here we show that melanoma cell proliferation in cultures with an ALI is higher than melanoma cell proliferation in submerged cultures. Bromodeoxyuridine (BrdU) uptake, an indicator of cell proliferation, of melanoma cells at the ALI was about 3 times that of submerged cells, while ALI and submerged melanoma cells had similar levels of single-stranded DNA (a marker of apoptosis). The ALI enhanced the expression of Raf-1, MEK-1 and pERK-1/2 components of the mitogen-activated protein kinase (MAPK) cascade, in cells more than the submerged condition did. The increases in BrdU uptake and pERK-1/2 expression promoted by ALI was abolished by the MEK inhibitor, PD-98059. ALI-treated and submerged melanoma cells did not infiltrate into the collagen gel, and they showed no significant difference in the expression of the invasion- and motility-related molecules, matrix metalloproteinase-1 and -9, laminin 5, and filamin A. Our data indicate that the ALI, a skin microenvironment, accelerates the growth, but not the apoptosis or invasion, of melanoma cells through MAPK activation.

Keywords: air-liquid interface; collagen gel invasion assay; melanoma; mitogen-activated protein kinase; proliferation

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