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Kathirvel K, Parija SC. Role of Na-K ATPase enzyme in vascular response of goat ruminal artery. Indian J Pharmacol. 2009;41(2):68-71doi: 10.4103/0253-7613.51343.
Kathirvel, K., & Parija, S. C. (2009). Role of Na-K ATPase enzyme in vascular response of goat ruminal artery. Indian journal of pharmacology, 41(2), 68-71. https://doi.org/10.4103/0253-7613.51343
Kathirvel, K, and Parija, S C. "Role of Na-K ATPase enzyme in vascular response of goat ruminal artery." Indian journal of pharmacology vol. 41,2 (2009): 68-71. doi: https://doi.org/10.4103/0253-7613.51343
Kathirvel K, Parija SC. Role of Na-K ATPase enzyme in vascular response of goat ruminal artery. Indian J Pharmacol. 2009 Apr;41(2):68-71. doi: 10.4103/0253-7613.51343. PMID: 20336220; PMCID: PMC2841235.
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Indian J Pharmacol. 2009 Apr;41(2):68-71. doi: 10.4103/0253-7613.51343.

Role of Na-K ATPase enzyme in vascular response of goat ruminal artery.

Indian journal of pharmacology

K Kathirvel, S C Parija

Affiliations

  1. Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar - 751 003, India.

PMID: 20336220 PMCID: PMC2841235 DOI: 10.4103/0253-7613.51343

Abstract

OBJECTIVE: To study the role of Na(+), K(+)- ATPase enzyme in the vascular response of goat ruminal artery.

MATERIALS AND METHODS: Ruminal artery was obtained in chilled aerated modified Krebs-Henseleit solution (KHS) from a local slaughterhouse and transported in ice for further processing. The endothelium intact arterial ring was mounted in a thermostatically controlled (37 +/- 0.5 degrees C) organ bath containing 20 ml of modified KHS (pH 7.4) bubbled with oxygen (95%) and CO(2) (5%) under 2g tension. An equilibration of 90 min was allowed before addition of drugs into the bath. The responses were recorded isometrically in an automatic organ bath connected to PowerLab data acquisition system. In order to examine intact functional endothelium, ACh (10 muM) was added on the 5-HT (1.0 muM) - induced sustained contractile response. Similarly, functional characterization of Na(+), K(+)-ATPase activity was done by K(+)-induced relaxation (10 muM-10 mM) in the absence and presence of ouabain (0.1 muM/ 0.1 mM), digoxin (0.1 muM) and barium (30 muM).

RESULTS: ACh (10(-5) M) did not produce any relaxing effect on 5-HT-induced sustained contractile response suggesting that vascular endothelium has no significant influence on the activation of sodium pump by extracellular K(+) in ruminal artery. Low concentration of Ba(2+) (30 muM) (IC(50): 0.479 mM) inhibited K(+)-induced relaxation suggesting K(ir) (inward rectifier) channel in part had role in K(+)-induced vasodilatation in ruminal artery. Vasorelaxant effect of KCl (10 muM-10 mM) in K(+)-free medium is also blocked by ouabain (0.1 muM and 0.1 mM) (IC(50):0.398 mM and IC(35): 1.36 mM), but not by digoxin (0.1 muM) (IC(50) 0.234 mM) suggesting that ouabain sensitive Na(+), K(+)-ATPase isoform is present in the ruminal artery.

CONCLUSION: In the goat ruminal artery functional regulation of sodium pump is partly mediated by K(+) channel and ouabain sensitive Na(+), K(+) ATPase.

Keywords: Barium; K+ channel; K+- ATPase; Na+; oubain; ruminal artery

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