Display options
Cite
Seismann H, Blank S, Cifuentes L, et al. Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity. Clin Mol Allergy. 2010;8:7doi: 10.1186/1476-7961-8-7.
Seismann, H., Blank, S., Cifuentes, L., Braren, I., Bredehorst, R., Grunwald, T., Ollert, M., & Spillner, E. (2010). Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity. Clinical and molecular allergy : CMA, 87. https://doi.org/10.1186/1476-7961-8-7
Seismann, Henning, et al. "Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity." Clinical and molecular allergy : CMA vol. 8 (2010): 7. doi: https://doi.org/10.1186/1476-7961-8-7
Seismann H, Blank S, Cifuentes L, Braren I, Bredehorst R, Grunwald T, Ollert M, Spillner E. Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity. Clin Mol Allergy. 2010 Apr 01;8:7. doi: 10.1186/1476-7961-8-7. PMID: 20359368; PMCID: PMC2867971.
Copy Download .nbib Format: NLM
Share it on

Clin Mol Allergy. 2010 Apr 01;8:7. doi: 10.1186/1476-7961-8-7.

Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity.

Clinical and molecular allergy : CMA

Henning Seismann, Simon Blank, Liliana Cifuentes, Ingke Braren, Reinhard Bredehorst, Thomas Grunwald, Markus Ollert, Edzard Spillner

Affiliations

  1. Institute of Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, Germany. [email protected].

PMID: 20359368 PMCID: PMC2867971 DOI: 10.1186/1476-7961-8-7

Abstract

BACKGROUND: Hymenoptera venoms are known to cause life-threatening IgE-mediated anaphylactic reactions in allergic individuals. Proper diagnosis of hymenoptera venom allergy using venom extracts is severely affected by molecular cross-reactivities. Although non-glycosylated marker allergens would facilitate the identification of the culprit venom, the major allergen phospholipase A1 (Ves v 1) from yellow jacket venom (YJV) remained unavailable so far.

METHODS: Expression of Ves v 1 as wild type and enzymatically inactivated mutant and Ves v 5 in insect cells yielded soluble proteins that were purified via affinity chromatography. Functionality of the recombinant allergens was assessed by enzymatic and biophysical analyses as well as basophil activation tests. Diagnostic relevance was addressed by ELISA-based analyses of sera of YJV-sensitized patients.

RESULTS: Both major allergens Ves v 1 and Ves v 5 could be produced in insect cells in secreted soluble form. The recombinant proteins exhibited their particular biochemical and functional characteristics and were capable for activation of human basophils. Assessment of IgE reactivity of sera of YJV-sensitized and double-sensitized patients emphasised the relevance of Ves v 1 in hymenoptera venom allergy. In contrast to the use of singular molecules the combined use of both molecules enabled a reliable assignment of sensitisation to YJV for more than 90% of double-sensitised patients.

CONCLUSIONS: The recombinant availability of Ves v 1 from yellow jacket venom will contribute to a more detailed understanding of the molecular and allergological mechanisms of insect venoms and may provide a valuable tool for diagnostic and therapeutic approaches in hymenoptera venom allergy.

References

  1. Clin Exp Allergy. 2005 Apr;35(4):441-7 - PubMed
  2. Allergy. 2002 Jul;57(7):570-6 - PubMed
  3. Int Arch Allergy Immunol. 2002 Oct;129(2):160-8 - PubMed
  4. Hautarzt. 2008 Mar;59(3):194-9 - PubMed
  5. Int Arch Allergy Immunol. 2000 Oct;123(2):99-106 - PubMed
  6. Allergy. 2009 Apr;64(4):543-8 - PubMed
  7. J Biol Chem. 2000 Apr 14;275(15):11451-8 - PubMed
  8. Mol Immunol. 1983 Mar;20(3):297-308 - PubMed
  9. Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M. 1999;(93):181-8 - PubMed
  10. J Immunol. 2010 May 1;184(9):5403-13 - PubMed
  11. Glycoconj J. 1999 Jun;16(6):271-81 - PubMed
  12. J Allergy Clin Immunol. 1996 Sep;98(3):588-600 - PubMed
  13. Int Arch Allergy Immunol. 2000 Apr;121(4):284-91 - PubMed
  14. Int Arch Allergy Immunol. 2002 Dec;129(4):286-95 - PubMed
  15. Allergy. 2006 Oct;61(10):1220-9 - PubMed
  16. Curr Opin Allergy Clin Immunol. 2003 Aug;3(4):299-303 - PubMed
  17. Mol Immunol. 2010 Jan;47(4):799-808 - PubMed
  18. Protein Expr Purif. 2006 Jun;47(2):621-8 - PubMed
  19. J Allergy Clin Immunol. 1995 Jun;95(6):1229-35 - PubMed
  20. Allergy. 2005 Nov;60(11):1339-49 - PubMed

Publication Types