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Liu J, Conboy JC. Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy. Vib Spectrosc. 2009;50(1):106-115doi: 10.1016/j.vibspec.2008.09.004.
Liu, J., & Conboy, J. C. (2009). Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy. Vibrational Spectroscopy, 50(1), 106-115. https://doi.org/10.1016/j.vibspec.2008.09.004
Liu, Jin, and Conboy, John C. "Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy." Vibrational Spectroscopy vol. 50,1 (2009): 106-115. doi: https://doi.org/10.1016/j.vibspec.2008.09.004
Liu J, Conboy JC. Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy. Vib Spectrosc. 2009 May 26;50(1):106-115. doi: 10.1016/j.vibspec.2008.09.004. PMID: 20361007; PMCID: PMC2846528.
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Vib Spectrosc. 2009 May 26;50(1):106-115. doi: 10.1016/j.vibspec.2008.09.004.

Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy.

Vibrational Spectroscopy

Jin Liu, John C Conboy

Affiliations

  1. University of Utah, Department of Chemistry, 315 S. 1400 E. RM 2020, Salt Lake City, Utah, 84112.

PMID: 20361007 PMCID: PMC2846528 DOI: 10.1016/j.vibspec.2008.09.004

Abstract

The influence of cholesterol (CHO) on the phase behavior of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) planar supported lipid bilayers (PSLBs) was investigated by sum-frequency vibrational spectroscopy (SFVS). The intrinsic symmetry constraints of SFVS were exploited to measure the asymmetric distribution of phase segregated phospholipid domains in the proximal and distal layers of DSPC + CHO binary mixtures as a function of CHO content and temperature. The SFVS results suggest that cholesterol significantly affects the phase segregation and domain distribution in PSLBs of DSPC in a concentration dependent manner, similar to that found in bulk suspensions. The SFVS spectroscopic measurements of phase segregation and structure change in the binary mixture indicate that membrane asymmetry must be present in order for the changes in SFVS signal to be observed. These results therefore provide important evidence for the delocalization and segregation of different phase domain structures in PSLBs due to the interaction of cholesterol and phospholipids.

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