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Funkenstein B, Skopal T, Rapoport B, et al. Characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata). Comp Biochem Physiol Part D Genomics Proteomics. 2007;2(3):187-99doi: 10.1016/j.cbd.2007.04.001.
Funkenstein, B., Skopal, T., Rapoport, B., Rebhan, Y., Du, S. J., & Radaelli, G. (2007). Characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata). Comparative biochemistry and physiology. Part D, Genomics & proteomics, 2(3), 187-99. https://doi.org/10.1016/j.cbd.2007.04.001
Funkenstein, B, et al. "Characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata)." Comparative biochemistry and physiology. Part D, Genomics & proteomics vol. 2,3 (2007): 187-99. doi: https://doi.org/10.1016/j.cbd.2007.04.001
Funkenstein B, Skopal T, Rapoport B, Rebhan Y, Du SJ, Radaelli G. Characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata). Comp Biochem Physiol Part D Genomics Proteomics. 2007 Sep;2(3):187-99. doi: 10.1016/j.cbd.2007.04.001. Epub 2007 Apr 11. PMID: 20483292.
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Comp Biochem Physiol Part D Genomics Proteomics. 2007 Sep;2(3):187-99. doi: 10.1016/j.cbd.2007.04.001. Epub 2007 Apr 11.

Characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (Sparus aurata).

Comparative biochemistry and physiology. Part D, Genomics & proteomics

B Funkenstein, T Skopal, B Rapoport, Y Rebhan, S J Du, G Radaelli

Affiliations

  1. Department of Marine Biology & Biotechnology, Israel Oceanographic & Limnological Research, P.O. Box 8030, Tel-Shikmona, Haifa 31080, Israel.

PMID: 20483292 DOI: 10.1016/j.cbd.2007.04.001

Abstract

The promoter region ( approximately 1400 bp) of myosin light chain 2 gene of fast skeletal muscle from the marine fish Sparus aurata was cloned, sequenced and characterized. It contains a consensus sequence for TATA box, six perfect E-boxes known as binding sites to myogenic basic helix-loop-helix transcription factors and four putative MEF2-binding sites. Three genomic fragments (truncated at their upstream region) of 244, 650 and 1400 bp showed promoter activity evidenced by muscle-specific reporter gene activity using transient expression of green fluorescent protein in microinjected zebrafish embryos and in skeletal muscle of S. aurata fry following intramuscularly injection of plasmid DNA. The three genomic fragments also directed luciferase activity in skeletal muscle of S. aurata fry following intramuscularly injection of plasmid DNA showing a 60 to 150-fold higher luciferase activity compared to that obtained with pGL3-Basic. These experiments show that the three genomic fragments are functional muscle-specific promoters which will be useful for directing myostatin and follistatin expression in fish muscle in order to study their effect on fish muscle growth.

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